中国现代医学杂志2018，Vol.28Issue(3)：21-27,7.DOI:10.3969/j.issn.1005-8982.2018.03.005
中药川贝对哮喘模型小鼠气道炎症的影响及可能机制研究
Effect of Bulbus Fritillariae Cirrhosae on airway inflammation in mouse model of asthma
摘要
Abstract
Objective To investigate the effect of Bulbus Fritillariae Cirrhosae (BFC) on airway inflammation in mouse model of asthma and potential mechanism. Methods BALB/C mice were randomly divided into normal group, model group, high dose group and low dose group. Mice were challenged with ovalbumin (OVA) to establish asthma model. BFC was orally administered at the dose of 18.0 mg/kg (high dose) and 9.0 mg/kg (low dose) while animals in normal group and model group were orally administered with normal saline. All drugs were administered for consecutive 28 days on a daily basis. Leukocyte counts in bronchoalveolar lavage fluid (BALF) were identified. Morphological abnormality and grading of tissue inflammation were determined. Concentration of IL-8 and IL-13 in serum was measured by Enzyme linked immunosorbent assay (ELISA). Expressions of CXCR-2, GRO-α, ENA-78 and NAP-2 positive cells were detected by Immunochemistry. Levels of CXCR-2, GRO-α, ENA-78 and NAP-2 mRNA were quantified by Real-time PCR. Results Neutrophils, macrophage, monocyte and eosinophils in BALF and inflammation score in model group increased significantly when compared with normal group (P < 0.05), which were attenuated by BFC treatment (high dose and low dose) (P < 0.05). Levels of IL-8 and IL-13 enhanced significantly in model group when compared with normal group (P < 0.05), which was ameliorated by BFC (P < 0.05). Expressions of CXCR-2, GRO-α, ENA-78, and NAP-2 were increased significantly in model group (P < 0.05) when compared with normal group, which was decreased by BFC (P < 0.05). Conclusion BFC exerts anti-inflammatory effect in asthmatic mice through deduction of IL-8, IL-13, CXCR-2 and GRO-α, ENA-78 and NAP-2.关键词
中药川贝/哮喘/气道炎症/趋化因子Key words
bulbus fritillariae cirrhosae/asthma/airway inflammation/chemokine分类
医药卫生引用本文复制引用
李厚忠,高照渝,王慧,任公平,徐红纳,刘艺,巩丽虹,张羽飞..中药川贝对哮喘模型小鼠气道炎症的影响及可能机制研究[J].中国现代医学杂志,2018,28(3):21-27,7.基金项目
黑龙江省自然科学基金(No:H201490) （No:H201490）
