中国畜牧杂志2017，Vol.53Issue(12)：29-33,5.DOI:10.19556/j.0258-7033.2017-12-029
伊犁马GHR基因多态性及其与泌乳性能的关联分析
Polymorphism of GHR Gene and Its Correlation with Lactation Performance in Yili Horse
摘要
Abstract
Mare's milk has a high nutritional value,but there are few research reports on lactation performance of horse until now.This trial was conducted to study the polymorphism of growth hormone receptor (GHR) gene in Yili horse and its relationship with lactation performance.13 pairs of primers were designed for the exons and promoter of GHR gene of Yili horse.DNA pooling was used as the template,and PCR product was directly sequenced.PCR-RFLP method was used to genotype the polymorphic sites in 188 Yili horses,and then the relationship between genotypes and lactation traits based on 73 of them was analyzed.The result showed that a T-562C mutation in GHR gene promoter was detected in Yili horse.The frequencies of genotype CC,TC and TT were respectively 0.309,0.66 and 0.016,and C allele was dominant and its frequency was 0.646.The frequency of T gene was 0.354.The results showed that although the milk yield of each genotype was not significantly different (P＞0.05),it was found that there seems to be a trend in the milk production of each genotype (TT＞ CC＞ CT).The mutation (C＞T) could increase the milk yield.The results can provide an identifiable ground for the Chinese dairy horse breeding efficiently.关键词
伊犁马/生长激素受体基因/泌乳性能/多态性Key words
Yili horse/GHR gene/Lactation performance/Polymorphism分类
农业科技引用本文复制引用
孟喆,于农淇,高源,邬明丽,樊英智,雷初朝,刘武军,党瑞华..伊犁马GHR基因多态性及其与泌乳性能的关联分析[J].中国畜牧杂志,2017,53(12):29-33,5.基金项目
国家自然科学基金项目(81270439) （81270439）
引进人才科研启动费(Z111021202) （Z111021202）
