中国畜牧杂志2017，Vol.53Issue(12)：67-72,6.DOI:10.19556/j.0258-7033.2017-12-067
高通量测序分析酵母培养物对肉仔鸡盲肠菌群的影响
Effect of Different Fermentation Duration of Yeast (Saccharomyces cerevisiae) Culture on the Intestinal Microbiota by Miseq High-throughput Sequencing
摘要
Abstract
This study was conducted to investigate the effect of different fermentation time of Yeast (Saccharomyces cerevisiae) Culture on the intestinal microbiota.One-day-old AA 336 broilers with the comparable relative quality were randomly assigned to 7 groups with 6 replicates of 8 broilers each.The broilers in the control treatment A were only fed a basal diet,and treatment B,C,D,E,F were yeast culture groups,supplemented with fermentation time at 12 h,24 h,36 h,48 h and 60 h of yeast culture,and treatment G was Diamond V XP,respectively.The experiment was based on 42 d to 7 d preliminary trial period.After 42 d.Cecum contents were collected and the intestinal microbiota was detected by Miseq high-throughput sequencing technology.The results showed as follow:Compared with the control group,at 7～42 d,averaged daily gain (ADG)in C and D group were significantly higher (P＜0.05),and that two groups of feed to gain ratio(F/G)were significantly lower.D group had the highest intestinal microbiota diversity while C group,exhibited the lowest species diversity.In the phylum level,Firmicutes was the most abundant bacterial in 7 groups,while the proportion of Verrucomicrobia was higher in C group was against the other group.In the genus level,Bacteroides,Faecalibacterium,lactobacillus,Blautia were the more abundant bacterial in 7 groups.In conclusion,different time of fermentation of yeast culture has a significant effect on intestinal flora,and thus we can control composition metabolites of yeast culture by adjusting the fermentation time,in turn,adjusting the structure of intestinal flora,affect the production performance of animal.关键词
酵母培养物/盲肠菌群/高通量测序Key words
Yeast culture(YC)/Intestinal microbiota/High-throughput sequencing technology分类
农业科技引用本文复制引用
孙喆,甄玉国,赵巍,赵小丽,王涛,秦贵信..高通量测序分析酵母培养物对肉仔鸡盲肠菌群的影响[J].中国畜牧杂志,2017,53(12):67-72,6.基金项目
吉林省教育厅“十二五”科学技术研究项目(2015181)) （2015181）
