中国畜牧杂志2017，Vol.53Issue(12)：79-85,7.DOI:10.19556/j.0258-7033.2017-12-079
体外法研究酵母发酵饲料对瘤胃发酵参数及瘤胃细菌数量的影响
Study on Effects of Yeast Fermented Feed on Rumen Fermentation Parameters and Rumen Bacteria in vitro Method
摘要
Abstract
In this study,the effects of diet added with yeast fermented feed on the ruminal fermentation parameters and the number of ruminal bacteria were studied in vitro.The experiment was divided into two groups-the control group only contained the basic culture substrate,while the experimental group also added yeast fermented feed.The total number of ruminal bacteria and six kinds of functionalruminal bacteria were determined by real-time fluorescence quantitative PCR (qPCR).Besides,we also determined the fermentation parameterssuch as gas production,pH value and volatile fatty acid (VFA).The results showed that the yeast fermented feed could increase the total number of ruminal bacteria (P＜0.05),and promote the growth and reproduction of Butyrivibriomfibrisolvens,Megasphaera elsdenii,Ruminococcus flavefaciens,Ruminococcus albus and Fibrobacter succinogenes (P＜0.05),but reduce the number of bovine streptococci significantly (P＜0.05).Comparing with the control group,the gas production of the experimental group increased significantly(P＜0.05).The addition of yeast fermented feed also significantly raised the concentration of ammonia nitrogen (NH3-N) (P＜0.05),and the concentration of bacterial protein (BCP)(P＜0.05).The content of acetic acid,butyric acid and total volatile fatty acid (TVFA)in the experimental group were significantly higher than those in the control group (P＜0.05).While the yeast feed had no significant influence on pH at each time point (P＞0.05).关键词
酵母发酵饲料/瘤胃细菌/体外法/qPCR/瘤胃发酵参数Key words
Yeast fermented feed/Ruminal bacteria/In vitro/qPCR/Ruminal fermentation parameters分类
农业科技引用本文复制引用
张棋炜,张政,杨晶晶,高民,胡红莲,刘大程..体外法研究酵母发酵饲料对瘤胃发酵参数及瘤胃细菌数量的影响[J].中国畜牧杂志,2017,53(12):79-85,7.基金项目
国家自然科学基金项目(31260560) （31260560）
内蒙古自治区科技计划项目(自治区应用技术研究与开发资金计划2016) （自治区应用技术研究与开发资金计划2016）
现代农业(奶牛)产业技术体系建设专项(CARS-37) （奶牛）
