中国畜牧杂志2018，Vol.54Issue(1)：34-37,4.DOI:10.19556/j.0258-7033.2018-01-034
蒙古马高负荷运动训练前后MSTN基因、CKM基因表达量分析
Analysis of the Expression of MSTN Gene and CKM Gene before and after High Load Exercise Training in Mongolian Horses
摘要
Abstract
In order to investigate the expression level of MSTN and CKM gene,before and after high load exercise Training in Mongolian horse.We estimated the expression stability in skeletal muscle of 4 commonly used reference genes (TTN,18s rRNA GAPDH,β-actin) with SYBR Green Ⅰ quantitative RT-PCR.After determined reference gene,we measured transcriptional levels ofMSTN gene and CKM gene.The results showed that the expression stability values in skeletal muscle of Mongolian horses were GAPDH(1.087)＞ β-actin (1.211)＞ TTN (1.285)＞ 18s rRNA (1.460)In turn,and the GAPDH gene was selected as the reference gene for its highest stability in this experiment.After high load exercise training,the mRNA expression of MSTN gene increased than training before,and CKM gene were decreased.This study laid the foundation for the gene research related to racing performance in Mongolian horses.关键词
蒙古马/高负荷运动/内参基因/MSTN/CKMKey words
Mongolian horse/High load exercise/Reference gene/MSTN/CKM分类
农业科技引用本文复制引用
康德措,其日格日,赵启南,赵一萍,芒来..蒙古马高负荷运动训练前后MSTN基因、CKM基因表达量分析[J].中国畜牧杂志,2018,54(1):34-37,4.基金项目
国家自然科学基金项目(31472070) （31472070）
内蒙古自治区高等学校科学研究项目(NJZY0701) （NJZY0701）
内蒙古农业大学科技成果转化项目(CGZH2015001) （CGZH2015001）
