中国畜牧杂志2018，Vol.54Issue(1)：103-107,5.DOI:10.19556/j.0258-7033.2018-01-103
猪A-FABP基因真核表达载体的构建及其组织表达
Constructing of Eukaryotic Expressing Vector and Tissues Expression of A-FABP Gene in Pig
摘要
Abstract
The longissimus dorsi muscle of Landrace pig was as experimental material,RNA was extracted and A-FABP gene was cloned,the eukaryotic expression vector of pEGFP-N1-A-FABP was constructed and transfected to fibroblasts by liposome,the fluorescence expression was detected after 48 h.Then the differential expression ofA-FABP gene in different tissues (heart,liver,spleen,lung,kidney,longissimus dorsi muscle and leg muscle) was detected by Q-PCR.Results showed that the pEGFP-N1-A-FABP fusion expression vector was successfully constructed,the fluorescence expression was successfully expressed in fibroblasts.The expression ofA-FABP gene in 7 different tissues was different.The gene expression in longissimus dorsi muscle and leg muscle were the highest compared with others,they had extremely significant difference (P＜0.01),the expression in spleen and kidney were significantly different from that of heart,liver and lung (P＜0.05),while the A-FABP gene was not significantly different in heart,liver and lung.Results indicated that A-FABP gene was expressed in eukaryotic vector and cells,and its expression had difference in different tissues of pigs.关键词
猪/A-FABP基因/真核表达载体/组织表达Key words
Pig/A-FABP gene/Eukaryotic expressiong vector/Tissues expression分类
农业科技引用本文复制引用
李晓玲,于光辉,崔小荣,张廷荣,孙金海..猪A-FABP基因真核表达载体的构建及其组织表达[J].中国畜牧杂志,2018,54(1):103-107,5.基金项目
国家转基因生物新品种培育科技重大专项(2016ZX08006-003) （2016ZX08006-003）
山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-13) （SDAIT-08-13）
