中国医学科学院学报2017，Vol.39Issue(6)：756-761,6.DOI:10.3881/j.issn.1000-503X.2017.06.004
长链非编码RNA CASC19在结直肠癌中的表达及功能
Expression and Function of Long Non-coding RNA CASC19 in Colorectal Cancer
摘要
Abstract
Objective To investigate the expression,function and significance of long non-coding RNA (lncRNA) CASC19 in colorectal cancer (CRC).Methods Real-time quantitative polymerase chain reaction was employed to determine the expression of CASC19 in 40 paired samples from CRC surgical specimens and 5 CRC cell lines.The correlations of CASC19 expression with clinicopathologic parameters were analyzed.Transwell assay was applied to detect the migration ability of CRC cells after the CASC19 expression was knocked down by small interfering RNA.Results The expression of CASC19 in colorectal cancer was significantly higher than those in adjacent normal mucosa tissues (t =5.527,P ＜ 0.000 1) and was associated with metastasis (P =0.044).Knockdown of CASC19 expression in CRC inhibited the migration ability of CRC in vitro.Conclusions The expression of CASC19 increases in CRC.CASC19 expression is not associated with age,gender,or tumor site/differentiation but with tumor size,lymph node metastasis,and distant metastasis,suggesting high CASC19 expression may promote CRC metastasis.关键词
CASC19/结直肠癌/迁移/长链非编码RNAKey words
CASC19/colorectal cancer/migration/long non-coding RNA分类
医药卫生引用本文复制引用
王健君,李晓敏,何雷,钟树志,彭彦霄,季娜..长链非编码RNA CASC19在结直肠癌中的表达及功能[J].中国医学科学院学报,2017,39(6):756-761,6.基金项目
皖南医学院重点科研项目培育基金(WK2016Z01)、皖南医学院中青年科研基金自然科学类(WK201619)和安徽省高校自然科学基金(KJ2017A250)Supported by the Wannan Medical College Key Research Projects(WK2016Z01),the Natural Sciences Youth Foundation of Wannan Medical College (WK201619),and the Natural Science Foundation of the Anhui Provincial High-Education Institutions (KJ2017A250) （WK2016Z01）
