中国医学科学院学报2017，Vol.39Issue(6)：817-826,10.DOI:10.3881/j.issn.1000-503X.2017.06.014
同位素标记相对和绝对定量蛋白组学技术筛选儿童哮喘不同病情控制水平血清差异蛋白
Screening Serum Differential Proteins for Childhood Asthma at Different Control Levels by Isobaric Tags for Relative and Absolute Quantification-based Proteomic Technology
摘要
Abstract
Objective To screen serum differential proteins for childhood asthma at different control levels,which provided the basis for the prevention and treatment of childhood asthma.Methods Isobaric tags for relative and absolute quantification,two-dimensional liquid chromatography,nanoelectrospray ionization,and high-resolution tandem mass spectrometry using the hybrid quadrupole time-of-flight platform was used to screen the differential proteins in serum samples from pediatric patients with controlled,partly controlled,or uncontrolled childhood asthma.Differential proteins were validated using enzyme-linked immunosorbent assay (ELISA).Results A total of 260 expressed proteins were identified.Among them 57 differentially expressed proteins were found among the different control levels of childhood asthma (fold ＜ 0.8 or fold ＞ 1.2).The differentially expressed proteins were involved mainly in 21 biological processes and 8 molecular functions and were located in 17 cellular components.ELISA showed that the serum vitronectin level was significantly higher in controlled group [(573.92 ±412.43) μg/ml] than in uncontrolled group [(382.27 ±238.64)] μg/ml (P =0.0399).Conclusion We identified 57 differential proteins for childhood asthma at different control levels,which may be used as potential biological targets for the control of childhood asthma.关键词
儿童哮喘/定量蛋白组学/差异性蛋白/质谱Key words
childhood asthma/quantitative proteomics/differential proteins/mass spectrometry分类
医药卫生引用本文复制引用
刘姬艳,王瑛,王远照,朱佳文,戴芳甸..同位素标记相对和绝对定量蛋白组学技术筛选儿童哮喘不同病情控制水平血清差异蛋白[J].中国医学科学院学报,2017,39(6):817-826,10.基金项目
浙江省科技计划项目(2015C33266)、杭州市科技发展计划项目(20150633B01)和国家自然科学基金(81403280)Supported by the Science and Technology Plan Project of Zhejiang Province (2015C33266),Hangzhou Science and Technology Development Plan Project (20150633B01),and the National Natural Sciences Foundation of China (81403280) （2015C33266）
