中国中医药信息杂志2018，Vol.25Issue(2)：69-73,5.DOI:10.3969/j.issn.1005-5304.2018.02.016
基于UPLC-Q-TOF/MS技术的桑叶化学成分快速识别分析
Fast Identification Analysis on Chemical Constituents in Mori Folium by Using UPLC-Q-TOF/MS Method
摘要
Abstract
Obejective To conduct fast identification analysis on chemical constituents of Mori Folium UPLC-Q-TOF/MS method.Methods ACQUITY UPLC-Q-TOF/MS spectrometer was used, acetonitrile and water (containing 0.1％ formic acid) as mobile phase with gradient elution. The flow rate was 0.3 mL/min, with ESI negative ion mode detection, data analysis with Masslynx4.1 software.Results According to the retention time provided by the mass spectrometry, the exact molecular weight and the secondary mass spectrometry were used to analyze the fragments by referring to literature, and 19 chemical constituents of Mori Folium were identified and deduced.Conclusion The method can analyze the chemical constituents of Mori Folium rapidly, sensitively and comprehensively, and provide the basis for the study of the pharmacological basis of Mori Folium.关键词
桑叶/超高液相色谱-四级杆-飞行时间质谱/化学成分/黄酮Key words
Mori Folium/UPLC-Q-TOF-MS/chemical constituents/flavonoids分类
医药卫生引用本文复制引用
刘金玲,李文姣,王韧,李森,张雨恬,周晋,贺福元..基于UPLC-Q-TOF/MS技术的桑叶化学成分快速识别分析[J].中国中医药信息杂志,2018,25(2):69-73,5.基金项目
国家自然科学基金(81573691) （81573691）
湖南省重点实验室建设项目(2016TP1017) （2016TP1017）
湖南省自然科学基金(2017JJ3236) （2017JJ3236）
湖南省教育厅科学研究项目(17B200) （17B200）
