中国中医药信息杂志2018，Vol.25Issue(2)：74-78,5.DOI:10.3969/j.issn.1005-5304.2018.02.017
芹菜素在Caco-2细胞的摄取研究
Uptake Study on Apigenin in Caco-2 Cells
摘要
Abstract
Objective To study the uptake property of apigenin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of apigenin on cell uptake in different times, concentrations, temperatures, pH values, and P-gp inhibitors. HPLC was used to determine the content of apigenin in cells.Results The linearity of apigenin concentration curve was found in good linear range of 0.33–80 μg/mL (r=0.9994). Uptake determination of apigenin in Caco-2 cells depended a certain of time, concentration and pH values, and P-gp inhibitors could not increase the apigenin uptake.Conclusion This method is simple and sensitive. P-gp is not involved in apigenin transport process, and uptake of apigenin in Caco-2 cells is mainly passive transported.关键词
芹菜素/Caco-2细胞/摄取/高效液相色谱法Key words
apigenin/Caco-2 cells/uptake/HPLC分类
医药卫生引用本文复制引用
郭闪,田春鸿,贺元,张晓霞,田莉..芹菜素在Caco-2细胞的摄取研究[J].中国中医药信息杂志,2018,25(2):74-78,5.基金项目
国家自然科学基金(81460590) （81460590）
