中国中医药信息杂志2018，Vol.25Issue(2)：79-82,4.DOI:10.3969/j.issn.1005-5304.2018.02.018
甘肃不同产地红芪中总黄酮及总多糖含量测定研究
Content Determination of Total Flavones and Polysaccharides in Hedysari Radix in Different Producing Areas in Gansu Province
摘要
Abstract
Objective To establish a method for content determination of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province.Methods The contents of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province were determined by ultraviolet spectroscopy with calycosin-7-glucoside and glucose as reference substance, and the wavelength was set at 260 nm and 484 nm.Results The contents were from 2.82 mg/g to 6.79 mg/g for total flavones and from 106.14 mg/g to 746.40 mg/g for total polysaccharides in Hedysari Radix in different producing areas in Gansu Province. The recoveries of total flavones and total polysaccharides were 97.96％ and 102.90％, respectively.Conclusion There was difference in contents of total flavones and polysaccharides of Hedysari Radix in different producing areas in Gansu Province, and the method of using ultraviolet spectroscopy is simple, reproducible, accurate and reliable, which can be preferably used as the method for content determination of total flavones and polysaccharides in Hedysari Radix.关键词
红芪/紫外分光光度法/毛蕊异黄酮苷/葡萄糖/总黄酮/总多糖Key words
Hedysari Radix/ultraviolet spectroscopy/calycosin-7-glucoside/glucose/total flavones/total polysaccharides分类
医药卫生引用本文复制引用
杨秀娟,杨志军,牛鹏贤,潘子昱,邵晶,李硕..甘肃不同产地红芪中总黄酮及总多糖含量测定研究[J].中国中医药信息杂志,2018,25(2):79-82,4.基金项目
甘肃省教育厅高等学校科研项目(2016B-055、2016B-054、2017A-056) （2016B-055、2016B-054、2017A-056）
甘肃省财政厅高等学校基本科研业务费项目(2013-1) （2013-1）
