中华临床免疫和变态反应杂志2017，Vol.11Issue(4)：344-350,7.DOI:10.3969/j.issn.1673-8705.2017.04.007
使用交链孢霉建立小鼠过敏性哮喘模型
Establishment of a Mouse Model of Alternaria-allergic Asthma
摘要
Abstract
Objective To investigate the feasibility of establishment of a mouse allergic asthma model by using Alternaria extract.Methods 24 Balb/c mice were divided into control and experimental group randomly , 12 in each group.The mice were given intranasal administration of Alternaria extract (25μg/ml, 50 μl) twice in experimental group or saline in control group under ether anesthesia at Day 4, 5, 6 and 7 in sensitization phase .Intranasal challenge was performed with Alternaria extract (50μg/ml) at Day 18, 19, 20 and Day 36, 37, 38 in experimental group and with saline in control group in the same procedure in challenge phase .The activity of mice was observed after last challenge .Airway responsiveness , total and differential cell count in bronchoalveolar lavage fluid ( BALF ) , serum total IgE and Alternaria specific IgE level , IL-4, IL-10 and IFN-γlevel in BALF and IL-25 mRNA, IL-33 mRNA expression in lung tissue were also detected after last challenge .The pathological changes of lung tissues were evaluated .Results The abnormal activities such as scratching , shortness of breath or restless were observed among mice in experimental group after challenge and recovery delayed , compared to the mice in control group.The airway resistance increased [ (0.87±0.17), (2.96±0.24), (10.57± 2.33), (13.26±5.79), (16.40±6.52) cm H2O/(ml· s), P<0.05 or P<0.01] and the airway compliance decreased [ (0.280±0.160), (0.150±0.092), (0.053±0.031), (0.046±0.022), (0.037± 0.009) ml/cm H2 O, P<0.05 or P<0.01 ] in experimental group .The total cell count [ ( 21.28 ± 4.58) ×105/ml], the percentage of eosinophils (68.33％±17.80％), serum total IgE [ (883.94± 122.33) ng/ml], specific IgE [ (65.76±15.56) ng/ml] and IL-4 [ (109.14±42.51) pg/ml] level in BALF and the expression level of IL-33 mRNA ( 2.21 ±0.58 ) in lung tissue was higher ( P<0.05 or P<0.01 ) , while the IFN-γ[ ( 93.42 ±51.26 ) pg/ml ] level was lower ( P<0.01 ) in experimental group.The expression of IL-25 mRNA was not detectable both in experimental and control group . Conclusion Balb/c mouse model of Alternaria-allergic asthma was successfully established , basing on the allergic inflammation profile shown in experimental group .关键词
模型,动物/哮喘/交链孢霉/气道反应性/白细胞介素25/白细胞介素33Key words
models,animal/asthma/Alternaria/airway responsiveness/interleukin 25/interleukin 33分类
医药卫生引用本文复制引用
李杰,王静茹,陈浩,杨林,祝戎飞..使用交链孢霉建立小鼠过敏性哮喘模型[J].中华临床免疫和变态反应杂志,2017,11(4):344-350,7.基金项目
国家自然科学基金(81401326) （81401326）
