浙江医学2017，Vol.39Issue(23)：2071-2074,2078,5.DOI:10.12056/j.issn.1006-2785.2017.39.23.2017-1547
同型半胱氨酸对C17.2小鼠神经干细胞的影响及作用机制
Effects of homocysteine on neural stem cells and its underlying mechanism
摘要
Abstract
Objective To investigate the effects of homocysteine (Hcy) on C17.2 mouse neural stem ceils (NSCS),and to explore its possible mechanism.Methods C17.2 mouse NSCs were cultured and divided into blank control group,Hcy group and Hcy+ N-acetylcysteine (NAC) group.The growth activity of NSCs was detected by CCK-8 method.The apoptosis of NSCs was assessed by flow cytometry analysis and caspase-3 method.Comet assay was used to evaluate the DNA damage of NSCs.The intracellular reactive oxidative species (ROS) level was detected by H2DCF-DA staining method.Results Hcy evoked ROS production and induced cell DNA damage,leading to an increase in NSCs apoptosis.On contrary,NAC decreased Hcy-evoked ROS production and significantly ameliorated DNA damage and improved NSCs survival.Conclusion Hcy may play a negative role in NSCs survival via inducing DNA damage by oxidative stress.关键词
同型半胱氨酸/DNA损伤/氧化应激/神经干细胞Key words
Homocysteine/DNA damage/Oxidative stress/Neural stem cell引用本文复制引用
王丹,阮妙华,周爱华,钱燕,陈亦明..同型半胱氨酸对C17.2小鼠神经干细胞的影响及作用机制[J].浙江医学,2017,39(23):2071-2074,2078,5.基金项目
国家自然科学基金项目(81701485) （81701485）
浙江省医药卫生科技计划项目(2017KY452) （2017KY452）
温州市科技局项目(2017Y0578) （2017Y0578）
