浙江医学2017，Vol.39Issue(23)：2084-2088,5.DOI:10.12056/j.issn.1006-2785.2017.39.23.2017-223
磷酸化4E-BP1在结直肠癌中的表达及与临床病理特征、预后的关系
Expression of phosphorylated 4E-BP1 and its relationship to prognosis in colorectal cancer
陈亦明 1王继生 1徐鲁白 1周斌 1范恒伟 1高静 1谢君青1
作者信息
- 1. 325027 温州医科大学附属第二医院肝胆外科
- 折叠
摘要
Abstract
Objective To evaluate the expression of phosphorylated eukaryotic translation initiation factor 4E-binding protein (p-4E-BP1) and to assess its relationship with clinicopathological features and prognosis in patients with colorectal cancer.Methods The expression of p-4E-BP1 in 164 specimens of colorectal cancer tissue was detected with immunohistochemistry.The expression of 4E-BP1 mRNA and protein in colorectal cancer and adjacent normal tissues were detected with qRT-PCR and Western bolt,respectively.Kaplan-Meier method and Cox proportional hazards regression model were used to analyze the survival of patients with colorectal cancer.Results p-4E-BP1 was positive in all colorectal cancer samples,including 95 (57.9％) cases with high expression and 69 (42.1％) cases with low expression.The expression level of p-4E-BP1 was significantly associated with tumor differentiation,invasive depth,lymph node metastasis and TNM stage.High expression of p-4E-BP1 was observed in colorectal cancer patients with poor differentiation,deeper infiltration,regional lymph node metastasis and later TNM stage (all P＜0.05).Although there were no significant differences in mRNA and protein expression of 4E-BP1 between tumor tissue and adjacent normal colorectal tissue,the phosphorylation level of 4E-BP1 was markedly increased in colorectal cancer tissue (P＜0.05).Kaplan-Meier survival analysis showed that the overall survival and progression-free survival of the p-4E-BP1 high expression group was significantly shorter than these of the low expression group(both P＜0.05).Cox proportional hazards model revealed that p-4E-BP1 was an independent adverse prognostic factor for both overall survival (RR=5.414,P＜0.05) and progression-free survival (RR=4.754,P＜0.05) in colorectal cancer patients.Conclusion High p-4E-BP1 expression is associated with tumor progression and adverse prognosis.p-4E-BP1 might serve as a novel biomarker to predict the clinical outcome of patients with colorectal cancer.关键词
结肠直肠癌/4E-BP1/磷酸化/临床病理特征/预后Key words
Colorectal cancer/4E-BP1/Phosphorylation/Clinicopathological features/Prognosis引用本文复制引用
陈亦明,王继生,徐鲁白,周斌,范恒伟,高静,谢君青..磷酸化4E-BP1在结直肠癌中的表达及与临床病理特征、预后的关系[J].浙江医学,2017,39(23):2084-2088,5.
