浙江医学2017，Vol.39Issue(24)：2197-2200,2221,5.DOI:10.12056/j.issn.1006-2785.2017.39.24.2017-823
miR-222通过MMP1对增生性瘢痕成纤维细胞的调控机制研究
MicroRNA-222 regulates the proliferation of fibroblasts in hypertrophic scar via matrix metalloproteinase 1
摘要
Abstract
Objective To determine the expressions of matrix metalloproteinase 1 (MMP1) and miR-222 in fibroblasts of hypertrophic scar (HS),and investigate the regulatory mechanism of miR-222 on the occurrence and development of HS.Methods HS tissues and HS-adjacent normal tissues were both collected from 36 HS patients.Primary fibroblasts were obtained.qRT-PCR was used to measure the level of mRNA of MMP1 and miR-222.Western blotting was carried out to determine MMP1 protein expression.MTT assay was employed to detect the proliferation of fibroblasts.Dual luciferase reporter assay was performed to identify the binding of miR-222 with MMP1 mRNA.Results MMP1 mRNA/protien were significantly downregulated and miR-222 was significantly upregulated in HS fibroblasts,compared with control fibroblasts (P＜0.05).The expression of MMP1mRNA/protein were significantly upregulated (P＜0.05),and the cell proliferation abilities was significantly inhibited in HS fibroblasts after transfected with pcDNAMMP1(P＜0.05).miR-222 was downregulated (P＜0.05),MMP1 mRNA was upregulated and the cell proliferation abilities was inhibited (P＜0.05)in HS fibroblasts after transfected with antagomiR-222,DuaI-Luciferase Reporter assay showed miR-222 regulated the expression of MMP1 by binding with its 3'-UTR.Conclusion miR-222 overexpressed in HS fibroblasts,miR-222 may regulate the proliferation and apoptosis of fibroblasts in HS via negatively regulating the expression of target gene MMP1.关键词
增生性瘢痕/microRNA-222/基质金属蛋白酶1Key words
Hypertrophic scar/MicroRNA-222/Matrix metalloproteinase 1引用本文复制引用
张谊,张璃,张启瑜,洪炜龙,林孝华..miR-222通过MMP1对增生性瘢痕成纤维细胞的调控机制研究[J].浙江医学,2017,39(24):2197-2200,2221,5.基金项目
浙江省医药卫生项目(2015KYB244) （2015KYB244）
