浙江医学2017，Vol.39Issue(24)：2205-2209,5.DOI:10.12056/j.issn.1006-2785.2017.39.24.2017-918
3型酸敏感离子通道在髓核致炎大鼠背根神经节中的表达研究
Effect of amiloride on ASIC3 expression in dorsal root ganglion following nerve root inflammation induced by nucleus pulposus in rats
摘要
Abstract
Objective To investigate the effect of amiloride on the expression of acid sensing ion channel 3 (ASIC3) in dorsal root ganglion (DRG) following nerve root inflammation induced by nucleus pulposus in rats.Methods Forty eight SD rats were randomly divided into control group(Sham,n=16),inflammation group (NP,n=16),inflammation+amiloride group (NP+Ami,n=16).The L5 DRG was exposed in rats of NP and NP+Ami groups by surgery,and sham surgery was performed in sham group.Intraperitoneal injection of amiloride 20μg/kg was given to rats in NP+Ami group q.d for 2 weeks after surgery,and no amiloride was given to rats in sham group and NP group.The mechanical pain withdrawal threshold (PWT) was evaluated at 2,4,6,8,10 and 12d after modeling.The expression of ASIC3,TNF-α,IL-6 mRNA and protein were examined by Real-time PCR and Western blot,respectively.TNF-α and IL-6 concentrations present in peripheral blood and DRG were determined at 1,3,7 and 14d after the surgical procedures using ELISA method.NO levels were examined by biochemistry test.Results Rats exposed to the NP showed decreased PWT at 12d after surgery (P＜0.01),and the levels of ASIC3 and the NO,TNF-α,IL-6 were up-regulated in DRG induced by NP 14d after surgery(all P＜0.01).Compared to NP group the mechanical PWT decreased at 8d after surgery and then gradually increased at 10 and 12d after surgery in NP+Ami group (all P＜0.01).ASIC3 was down-regulated (both P＜0.05),and the levels of NO,TNF-α,IL-6 were significantly decreased at 14d after surgery (all P＜0.01) in NP+Ami group.Conclusion ASIC3 may upgrade the expression of NO and inflammatory cytokines TNF-α and IL-6 to induce hyperalgesia and never root pain.关键词
椎间盘/髓核/3型酸敏感离子通道/炎症Key words
Intervertebral disc/Nucleus pulposus/ASIC3/Inflammation引用本文复制引用
林士明,陈亦鹏,潘浩,王栋,贾高永,胡庆丰..3型酸敏感离子通道在髓核致炎大鼠背根神经节中的表达研究[J].浙江医学,2017,39(24):2205-2209,5.基金项目
浙江省中医药科技计划项目(2015ZA157) （2015ZA157）
杭州市科技计划重点项目(20150733Q60) （20150733Q60）
