植物研究2018，Vol.38Issue(1)：56-63,8.DOI:10.7525/j.issn.1673-5102.2018.01.007
芍药愈伤组织中体细胞胚发育过程的组织细胞学观察
Histocytology Observation on the Somatic Embryogenesis in Herbaceous Peony Callus
摘要
Abstract
The stem segments,leaves and petioles of three herbaceous peony was used as explants for somatic embryogenesis.Paraffin method was used for morphological and histological observation of somatic embryogeneris.The main conclusions were as follows:callus induction rate from stems of'Going Bananas' was 100％.Proliferation rate of the callus was over 4.0.The best induction and proliferation medium of inducting callus wasWPM+IAA1.0mg· L-1 +6-BA 1.0 mg· L-1 +NAA0.5 mg· L-1+TDZ0.5 mg· L-1 +CH 0.625 g· L-1.Embryogenic callus generated after transferred the callus to 1/2 MS(Ca2+ doubled) +2,4-D 2.0 mg· L-1 + ABA 0.5 mg · L-1 or ZT 1.0 mg · L-1 medium of a constantly dark cultivation of 90 d.Globular embryos and heart embryos were generated after transferred the embryogenic callus to 1/2MS(Ca2+ doubled) +6-BA1.0 mg · L-1 + NAA 0.2 mg · L-1 medium for a constantly illumination cultivation of 60 d.There are exogenous and endogenous origins of somatic system.Exogenous includes origins from individual surface cells and origins from multiple subcritical cells.The difference of three ways lies mainly in starting position and starting cells number,they will all generated proembryo later.The splitting of embryogenic cells was symmetrical and the presence of asymmetric splitting cells was not found.关键词
芍药/体细胞胚/起源方式/组织细胞学Key words
herbaceous peony/somatic embryo/origin/histocytology分类
农业科技引用本文复制引用
魏冬霞,张滕,郑严仪,于晓南..芍药愈伤组织中体细胞胚发育过程的组织细胞学观察[J].植物研究,2018,38(1):56-63,8.基金项目
Graduate Training and Development Program of Beijing Municipal Commission of Education (BLCXY201629) （BLCXY201629）
National Natural Science Foundation of China(31400591)北京市教育委员会科学研究与研究生培养共建项目资助(BLCXY201629) （31400591）
国家自然科学基金(31400591) （31400591）
