植物研究2018，Vol.38Issue(1)：91-99,9.DOI:10.7525/j.issn.1673-5102.2018.01.011
短期盐胁迫下盐穗木的转录组分析
Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress
摘要
Abstract
As a halophyte with strong salt tolerance,Halostachys caspica widely distributes in desert salinealkali land.In order to reveal the genomic changes of gene expression under salt stress,transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol · L-1 NaC1 for 3 h was performed.A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled.The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways,respectively.Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt (300 mmol · L-1) and high salt (500 mmol · L-1) in short-term stress,respectively.The 1 245 unigenes were the common differentially expressed genes in the two salt stresses.They were mainly enriched in cellular process,metabolic process and response to stimulus.The osmotic regulation and reactive oxygen species (ROS) scavenging genes were screened out,and most of them were up-regulated.Therefore,H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.关键词
盐穗木/盐胁迫/转录组/差异基因/渗透调节/活性氧Key words
Halostachys caspica/salt stress/transcriptome/differentially expressed genes/osmotic adjustment/reactive oxygen species分类
农业科技引用本文复制引用
张丽丽,张富春..短期盐胁迫下盐穗木的转录组分析[J].植物研究,2018,38(1):91-99,9.基金项目
Supported by Special Funds of Xinjiang Key Laboratory (2014KL001)新疆重点实验室专项资金资助项目(2014KL001) （2014KL001）
