植物研究2018，Vol.38Issue(1)：155-160,6.DOI:10.7525/j.issn.1673-5102.2018.01.019
塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究
Study on Orthogonal Optimization and Antioxidation of Polysaccharide from Tara Seeds
摘要
Abstract
Tara seed as raw material,process and antioxidant properties of protein and polysaccharide of Tara seed polysaccharide of Tara.Using the loss rate of polysaccharide and protein removal rate as the evaluation index to compare Sevage method,three trichloroacetic acid method and papain method of Tara polysaccharide deproteinization effect.Removing protein were optimized by using orthogonal optimization experimental design principle and the method of orthogonal analysis of four factors and three levels of papain method.The results showed that:Tara polysaccharide best protein removal technology for enzyme dosage 0.15 mL,hydrolysis time 90 min,enzymolysis temperature of 60℃,enzymolysis pH =6,protein removal rate was 95.19％,rate of polysaccharide is 75.02％.Through the study of Tara polysaccharide antioxidation of polysaccharide retention at Tara,it is showed thatthe total antioxidant activity of sugar was better,which had a strong scavenging effect on DPPH free radical.关键词
塔拉多糖/脱蛋白/正交优化/抗氧化性Key words
Tara polysaccharide/removing protein/orthogonal optimization/antioxidant activity分类
农业科技引用本文复制引用
袁德成,赵寒梅,杨逢建..塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究[J].植物研究,2018,38(1):155-160,6.基金项目
Forestry Public Welfare Industry Research Special(201404616)林业公益性行业科研专项(201404616) （201404616）
