中国中西医结合杂志2018，Vol.38Issue(1)：80-84,5.DOI:10.7661/j.cjim.20170630.149
地榆皂苷Ⅰ诱导人甲状腺乳头状癌细胞BCPAP凋亡的实验研究
Ziyuglycoside Ⅰ Induced Apoptosis of Human Thyroid Papillary Carcinoma BCPAP Cells
摘要
Abstract
Objective To observe the effects and underlying molecular mechanisms of Ziyuglycoside Ⅰ (Z Ⅰ) on human thyroid papillary carcinoma BCPAP cells.Methods The proliferation inhibitory of Z Ⅰ on BCPAP cells was detected by Mlr assay.Cell apoptosis was detected by flow cytometry with double staining of Annexin V/PI.The expression levels of apoptosis-related proteins were performed by Western blot.Mitochondrial membrane potential (MMP) was detected by flow cytometry with JC-1 staining.Caspase activity was detected by reagent kit.Results Z Ⅰ significantly inhibited the proliferation of BCPAP cells in a dose-dependent manner (P ＜0.05,IC50 value:28.99 μmol/L).Z Ⅰ could positively induce BCPAP cell apoptosis in a dose-dependent manner.Z Ⅰ could increase Bax/Bcl-2 ratio,decrease the level of MMP,promote the release of cytochrome C from mitochondria,and elevate activities of Caspase-3 and Caspase-9.Conclusion Z Ⅰ could inhibit the proliferation of human thyroid papillary carcinoma BCPAP cells through activating mitochondria-dependent pathway and induce cell apoptosis.关键词
地榆皂苷Ⅰ/甲状腺乳头状癌/BCPAP细胞/凋亡Key words
Ziyuglycoside Ⅰ/thyroid papillary carcinoma/BCPAP cell/apoptosis引用本文复制引用
高芸,王柯,朱雪,潘懿,贾冰..地榆皂苷Ⅰ诱导人甲状腺乳头状癌细胞BCPAP凋亡的实验研究[J].中国中西医结合杂志,2018,38(1):80-84,5.基金项目
国家自然科学基金资助项目(No.81300787) （No.81300787）
