中国中西医结合杂志2018，Vol.38Issue(1)：91-96,6.DOI:10.7661/j.cjim.20171125.289
丹蒌片预防兔动脉粥样硬化支架置入术后再狭窄的作用及机制研究
Roles and Mechanism Study on Danlou Pill for Preventing In-Stent Restenosis of Atherosclerosis Rabbits
摘要
Abstract
Objective To observe roles and posible mechanism of Danlou Pill (DLP) for preventing bare metal stent (BMS) in-stent restenosis (ISR) of atherosclerosis rabbits,and to study potential mechanisms.Methods Abdominal aorta atherosclerosis model was established in 30 Japanese big ear rabbits by balloon injury and high-fat diet feeding after they took aspirin (25 mg/d) for 3 days.Four weeks later arteriography showed abdominal aorta atherosclerosis ＞30％ occurred in 24 rabbits.After implanting bare metal stents,the rabbits were randomly divided into 3 groups,the blank control group,the conventional treatment group,and the DLP group,8 in each group.Rabbits in the conventional treatment group were administered with aspirin [50 mg/(kg · d),clopidogrel [25 mg/(kg · d)],and rosuvastain [1 mg/(kg · d)] by gastrogavage.Rabbits in the DLP group were additionally administered with DLP [-0.5 g/(kg · d)] by gastrogavage.Equal volume of normal saline was administered to rabbits of the blank control group.The administration lasted for 4 successive weeks.Rabbits were examined by abdominal aorta (optical coherence mography,OCT) 4 weeks later.All stent-implanted arteries in these animals were evaluated by histology.Blood lipids and inflammatory factors were detected as well.HE staining was performed after sampling.p-AKT protein expression was detected by Western blot.Results At post-PCI 4 weeks,TG,TC,LDL-C,Hs-CRP,M and TNF-α all significantly decreased in the conventional treatment group and the DLP group,when compared with pre-PCI in the same group as well as the blank control group at the same time point (P ＜0.01).Moreover,TC levels were lower in the DLP group than in the conventional treatment group (P ＜0.05).OCT and HE staining analyses showed as compared with the blank control group,the hyperplasia of neointima was symmetric,the degrees of neointima hyperplasia and luminal stenosis were attenuated (all P ＜ 0.01).The effects of DLP was superior to those of the conventional treatment group,but with no statistical difference between the two groups (P ＞ 0.05).Western blot showed that p-Akt expression significantly decreased in the conventional treatment group and the DLP group,as compared with the blank control group (all P ＜0.01),but with no statistical difference between the former two groups (P ＞0.05).Conclusion DLP could prevent ISR after implanting bare metal stents possibly by lowering inflammatory reactions and inhibiting AKT signal pathways.关键词
动脉粥样硬化/支架/支架内再狭窄/丹蒌片Key words
atherosclerosis/stent/in-stent restenosis/Danlou Pill引用本文复制引用
王贺,李志海,邱承杰,司春婴,解金红,陈玉善,罗明华,朱明军,关怀敏..丹蒌片预防兔动脉粥样硬化支架置入术后再狭窄的作用及机制研究[J].中国中西医结合杂志,2018,38(1):91-96,6.基金项目
国家自然科学基金面上项目(No.81273949/H2902,No.81473507/H2902) （No.81273949/H2902,No.81473507/H2902）
河南省科技攻关项目(No.122102310191) （No.122102310191）
河南省教育厅高等学校重点科研项目(No.14A320051) （No.14A320051）
