中药材2017，Vol.40Issue(7)：1622-1627,6.DOI:10.13863/j.issn1001-4454.2017.07.027
桂枝指纹图谱的建立及识别模式研究
Chemical Fingerprint Establishment and Pattern Recognition for Cinnamomi Ramulus
摘要
Abstract
Objective:To establish chemical fingerprint and pattern recognition of total statistical moment method,cluster analysis and principal component analysis (PCA)for Cinnamomi Ramulus,and provide a method for quality control of Cinnamomi Ramulus.Methods:Chemical fingerprint of eleven batches of Cinnamomi Ramulus were established by HPLC,and total statistical molnent parameters,under cunrve of total quantum (AUCT),area under curve of total quantum perweight (AUCPWT),mean chromatographic retention time of total quantum(MCRTT),variance of chromatographic retention time of total quantum(VCRTT)were also calculated successfully.The similarities were obtained by total statistical moment method,angle cosine method and correlation coefficient method.Cluster analysis and PCA method were used to classify and recognize the samples.Results:Chemical fingerprint was established with eleven common peaks,and coumarin,cinnamic alcohol,cinnamic acid,cinnamic aldehyde,2-methoxy cinnamic aldehyde were identified.AUCT,AUCPWT,MCRTT,and VCRTT were calculated as 1.60 × 105 AU · min,5.38 × 102 AU · min · mL/mg,2.95 × 101 min,1.78 × 101 min2-,respectively.The similarities of all batches of samples were over 0.794.The samples were classified in to four categories bv cluster analysis and PCA method.Conclusion:It is feasible to establish chemical fingerprint which were recognized by total statistical moment method,cluster analysis,principal component analyvsis.关键词
桂枝/指纹图谱/总量统计矩/聚类分析/主成分分析Key words
Cinnamomi Ramulus/Fingerprint/Total statistical moment method/Cluster analysis/Principal component analysis分类
医药卫生引用本文复制引用
王元清,韩彬,陈婷,贺福元,严建业..桂枝指纹图谱的建立及识别模式研究[J].中药材,2017,40(7):1622-1627,6.基金项目
国家自然科学基金(81173194) （81173194）
湖南省教育厅科研项目(15C1432) （15C1432）
中南林业科技大学人才引进项目(2013RJ026) （2013RJ026）
湖南省中药活性物质筛选工程技术研究中心项目资助(湘科计字[2013]83号) （湘科计字[2013]83号）
湖南省“中药学”重点学科资助(湘教通[2011]76号) （湘教通[2011]76号）
