中药材2017，Vol.40Issue(8)：1753-1757,5.DOI:10.13863/j.issn1001-4454.2017.08.001
蒙古黄芪毛状根的高效诱导及毛状根生物量和总黄酮含量的比较分析
The High Efficient Induction of Hairy Roots of Astragalus membranaceus var.mongholicus and Comparative Analysis the Biomass and Total Flavonoids Content of Hairy Roots
摘要
Abstract
Objective:To solve the low induction rate of Astragalus membranaceus var.mongholicus hairy roots problem,and to analyze the biomass and the total flavonoids content of hairy roots,in order to lay the foundation for the utilization of Astragalus membranaceus var.mongholicus hairy roots.Methods:Hairy roots induction from leaves,petiole and stem of the natural growth of the young Astragalus membranaceus var.mongholicus were implemented through using Agrobacterium rhizogenes R1601 with different concentrations.Hairy roots were identified by PCR analysis,the induction rate was counted.The content of total flavonoids of hairy roots was determined by using ultraviolet spectrophotometric method.Results:The induction rate of three kinds of explants reached 79.3％ ～ 94.9％ with infection of four different concentrations of Agrobacterium rhizogenes R1601.The most suitable A60o value of Agrobacterium rhizogenes R1601 for the infection was 1.5,which could reach a maximum induction rate of hairy roots from leaves as 94.9％.PCR identification showed that the hairy roots were induced by Agrobacterium rhizogenes R1601.The biomass of different hairy root strains had significant difference.The difference of the total flavonoids content is not obvious in different strains of hairy root,but the total flavonoids of every strain was significantly higher than that of control.Conclusion:The efficient hairy roots induction system of Astragalus membranaceus var.mongholicus is established,and shows that the hairy root lines are screened by using the hairy roots of Astragalus membranaceus var.mongholicus for the synthesis of medicinal composition.关键词
蒙古黄芪/毛状根/生物量/总黄酮Key words
Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao/Hairy roots/Biomass/Total flavonoids分类
医药卫生引用本文复制引用
姚庆收,陈向明,马玉娥,刘晓云,林婷婷..蒙古黄芪毛状根的高效诱导及毛状根生物量和总黄酮含量的比较分析[J].中药材,2017,40(8):1753-1757,5.基金项目
山东省高校科技计划资助项目(J15LE51) （J15LE51）
