中药材2017，Vol.40Issue(8)：1758-1762,5.DOI:10.13863/j.issn1001-4454.2017.08.002
外源AgNO3对秦艽幼苗生长及龙胆苦苷生物合成的影响
Effect of Exogenous AgNO3 on the Growth and Gentiopicroside Accumulation of Gentiana macrophylla Seedlings
摘要
Abstract
Objective:To investigate the effects of exogenous AgNO3 on the growth and gentiopicroside accumulation of Gentiana macrophylla seedlings.Methods:Gentiana macrophylla seedlings were used to reserch,the concentrations of gentiopicroside in Gentiana macrophylla seedlings treated with 1 mmol/L AgNO3 were measured by HPLC method.The growth of the Gentiana macrophylla seedlings were observed after treated with AgNO3.The expression of key enzyme geneson and the gentiopicroside biosynthesis pathway were detected by Real time fluorescent quantitative PCR(RT-qPCR).Results:Compared with control group,the content of gentiopicroside in Gentiana macrophylla seedlings increased to 1.91 times,and the fresh weight,dry weight and root length were increased by an average of 29％,32％ and 43％ when they were treated with 1 mmol/L AgNO3 for 9 d.RT-qPCR results showed that the expression of some key enzyme geneson gentiopicroside biosynthesis pathway were significantly enhanced after treated with exogenous AgNO3.Conclusion:Exogenous AgNO3 can significantly promote the accumulation of gentiopicroside in Gentiana macrophylla seedlings,increase the biomass(dry weight and fresh weight)and root elongation of Gentiana macrophylla seedling.关键词
秦艽/AgNO3/龙胆苦苷/RT-qPCRKey words
Gentiana macrophylla Pall./AgNO3/Gentiopicroside/RT-qPCR分类
医药卫生引用本文复制引用
杨新兵,郭晓荣,王怀琴,周中枢,曹晓燕..外源AgNO3对秦艽幼苗生长及龙胆苦苷生物合成的影响[J].中药材,2017,40(8):1758-1762,5.基金项目
陕西省自然科学基金(2014JQ3107) （2014JQ3107）
中央高校基本科研业务费专项资金项目(GK201302043) （GK201302043）
陕西师范大学大学生创新创业训练计划项目(CX16029) （CX16029）
