中药材2017，Vol.40Issue(8)：1833-1837,5.DOI:10.13863/j.issn1001-4454.2017.08.018
驱虫斑鸠菊化学成分及酪氨酸酶抑制活性研究
Chemical Constituents and Tyrosinase Inhibition Activities of the fruits from Vernonia anthelmintica
摘要
Abstract
Objective:To study the chemical constituents of the fruits from Vernonia anthelmintica,and to study the effects of flavonoids on the activity of mushroom tyrosinase.Methods:The chemical constituents of 80％ ethanol extract of Vernonia anthelmintica were isolated and purified by various chromatographic methods including silica gel,Sephadex LH-20 column chromatography,and preparative HPLC.Their structures were elucidated on the basis of spectroscopic data and physico-chemical methods.The inhibitory activity of flavonoids against tyrosinase was evaluated by measuring the oxidation rate of DL-dopa.Results:Fourteen compounds were isolated from the fruits of Vernonia anthelmintica,and the structures were identified as N-acetylindole-3-carboxaldehyde(1),1-ethylindole-3-carboxylic acid (2),stigmasterol (3),eriodictyol (4),apigenin (5),butein (6),butin (7),isorhamnetin (8),sulphuretin (9),luteolin (10),caffeic acid (11),protocatechuic acid (12),3,5-O-dicaffeoylquinic acid methyl ester (13),3,4-O-dicaffeoylquinic acid methyl ester (14).Compound 9 was a strong inhibitor of mushroom tyrosinase,IC50 =0.424 mmol/L.Conclusion:Compounds 1,2,9,12 ～14 are i solated from this plant for the first time,compounds 1,2,9 are islolated from this genus for the first time.Compound 9 can be used as a potential natural tyrosinase inhibitor for further research.关键词
驱虫斑鸠菊/化学成分/黄酮/酪氨酸酶/抑制作用Key words
Vernonia anthelmintica (L.) Willd./Chemical constituents/Flavonoids/Tyrosinase/Inhibitory effect分类
医药卫生引用本文复制引用
王加利,潘纪红,袁将,姬瑞芳,全庆华,高简,刘永刚..驱虫斑鸠菊化学成分及酪氨酸酶抑制活性研究[J].中药材,2017,40(8):1833-1837,5.基金项目
国家自然科学基金(30901959) （30901959）
新疆自然科学基金(201318101-5) （201318101-5）
