中药材2017，Vol.40Issue(8)：1870-1875,6.DOI:10.13863/j.issn1001-4454.2017.08.027
韩信草UPLC指纹图谱及LC-MS化学成分分析
UPLC Fingerprint and LC-MS Chemical Constituents of Scutellaria indica
王领弟 1徐宝欣 2李艳荣 2潘海峰2
作者信息
- 1. 承德医学院附属医院药剂科,河北承德067000
- 2. 承德医学院河北省中药研究与开发重点实验室,河北承德067000
- 折叠
摘要
Abstract
Objective:To establish the UPLC fingerprint of Scutellaria indica,and infer the structure of the chemical composition in Scutellaria indica using LC-MS technology.Methods:UPLC method was taken on Agilent Poroshell 120 SB-C18 column(100 mm× 2.1 mm,2.7 μm),and the mixture of 0.1％ formic acid(A)-acetonitrile(B) were used as the mobile phase in a gradient elution model;the wavelength was 275 nm,the flow rate was 0.4 mL/min,the temperature was 35 ℃ and the sample size was 2 μL.In the positive and negative ion mode,the Triple TOF 5600 detector and the ESI source were used to determine the parameters of the mass spectra:the mass scan range was m/z 50 ～1 250 Da;the source temperature was 500 ℃;the atomization gas was 55 psi;the auxiliary gas was 55 psi;the air curtain gas was 35 psi;the ion scanning voltage was 5 500 V (positive ion mode),-4 500 V (negative ion mode).Results:32 common peaks were confirmed from twelve batches of Scutellaria indica UPLC fingerprint.Combining with the reference substance,literature and according to the corresponding fragments of cracking rule infference,27 flavonoids were determined.Conclusion:UPLC fingerprint of Scutellaria indica is established and the chemical components are identified by high resolution mass spectrometry,from the point of view of qualitative controls the quality of Scutellaria indica.关键词
韩信草/化学成分/液质联用Key words
Scutellaria indica Linn./Chemical composition/LC-MS分类
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王领弟,徐宝欣,李艳荣,潘海峰..韩信草UPLC指纹图谱及LC-MS化学成分分析[J].中药材,2017,40(8):1870-1875,6.
