中药材2017，Vol.40Issue(8)：1935-1939,5.DOI:10.13863/j.issn1001-4454.2017.08.042
茶条槭果对H22荷瘤小鼠的抑瘤作用
Study Anti-Tumor Effect of Acer ginnala Fruit on H22Tumor-Bearing Mice
摘要
Abstract
Objective:To study the inhibition and the mechanism of the two rough separation sites of Acer ginnala fruit in H22 tumor-bearing mice.Methods:H22 tumor-bearing mice were established by inoculating H22 mouse hepatoma cells in the left forelimb of the mice,and intraperitoneal administration drugs to observe 10 d,the tumor growth was also observed.The tumor inhibition rate was calculated,the levels of serum IL-6,IFN-γ,TNF-α,ALT,AST,BUN and CRE were measured by the kit to investigate the effects of the two rough separation sites on the H22 transplantation tumor growth inhibition.The pathological changes of the tumor tissues were observed by HE staining,and the apoptotic cells were detected by TUNEL method.Results:Two rough separation sites of Acer ginnala fruit could reduce the levels of IL-6,TNF-α and INF-γ,and decrease the levels of CRE,BUN,AST and ALT in the serum of H22 tumor bearing mice.Drugs groups were stained to observe the necrotic area.Conclusion:The results show that two rough separation sites of Acer ginnala fruit can significantly inhibit the growth of tunor cells,protect the liver and kidney,enhance the immune level,and inhibit the growth of tumor cells by inducing tumor cell apoptosis.关键词
茶条槭/茶条槭果/H22荷瘤/抗肿瘤/环磷酰胺Key words
Acer ginnala Maxim./Acer ginnala Maxim.fruit/H22 tumor/Antitumor/Cyclophosphamide分类
医药卫生引用本文复制引用
朱晓富,赵岩,蔡恩博,郜玉钢,杨鹤,刘双利,张连学..茶条槭果对H22荷瘤小鼠的抑瘤作用[J].中药材,2017,40(8):1935-1939,5.基金项目
国家公益性行业科研专项(201303111) （201303111）
吉林省科技发展计划项目(20140204013YY,20150307012YY,20160414008GH) （20140204013YY,20150307012YY,20160414008GH）
