中国现代中药2017，Vol.19Issue(10)：1367-1370,1381,5.DOI:10.13313/j.issn.1673-4890.2017.10.003
基于ITS2序列的博落回属植物鉴定研究
Identification of Plants in Macleaya spp.Based on ITS2 Sequence
摘要
Abstract
Objective:To identify the medicinal plant Macleaya spp.by using ITS2 sequence.Methods:The ITS2 sequence and sequence of 30 samples collected from Hunan,Jiangxi,Zhejiang,Hubei,Henan,Shanxi,Chongqing was amplified and the amplification efficiency,the sequencing success rate,the constitution of base pair,intra-and inter-specific variation and NJ(Neighbor-Joining) tree were analyzed,in order to evaluate the ITS2 sequencing results.Results:The ITS2 sequence length of Macleaya spp.was within 500 bp;the amplification efficiency and the success sequencing rate was 100％ and 30 ITS2 sequences were obtained.Conclusion:The ITS2 sequence can be effective and accurate for identification of Macleaya spp.关键词
博落回属/ITS2序列/DNA条形码/物种鉴定/遗传分化Key words
Macleaya spp./ITS2 sequence/DNA barcode/Species identification/Genetic differentiation引用本文复制引用
黄鹏,刘金凤,徐敏,夏丽琼,柳亦松,唐其,唐昭山,曾建国..基于ITS2序列的博落回属植物鉴定研究[J].中国现代中药,2017,19(10):1367-1370,1381,5.基金项目
国家重点实验室培育基地开放项目(15KFXM09) （15KFXM09）
湖南省科技重点计划(2016SK3002) （2016SK3002）
