中国现代中药2017，Vol.19Issue(10)：1376-1381,6.DOI:10.13313/j.issn.1673-4890.2017.10.005
基于UHPLC-QTOF/MS的博落回花中生物碱类化学成分研究
Identification of Alkaloids in Flowers of Macleaya cordata (Willd) R.Br.by UHPLC-QTOF/MS
摘要
Abstract
Objective:To elucidate the minor alkaloids in the flower of Macleaya cordata by using the ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) for safety evaluation of M.cordata.Methods:The alkaloids were found and identified through the serious data mining,structural speculation on basis of the careful analysis of the MS and MS/MS data,and confirmed by comparison with the reference standards and library.Results:There were total 22 alkaloids found in the flower of M.cordata including protopines and tetrahydroprotonerber ines,the main alkaloids including protopine,allocryptopine,N-methyltetrahydroberberine,N-methyltetrahydropapaverine.Conclusion:The results provide sufficient scientific evidence for evaluating its quality and safety of the flowers from M.cordata comprehensively and objectively.关键词
博落回花/生物碱/UHPLC-QTOF/MS/定性分析Key words
Flowers of Macleaya cordata/alkaloid/UHPLC-QTOF/MS/qualitative analysis引用本文复制引用
黄嘉璐,刘秀斌,郑亚杰,邹超兰,唐昭山,曾建国..基于UHPLC-QTOF/MS的博落回花中生物碱类化学成分研究[J].中国现代中药,2017,19(10):1376-1381,6.基金项目
国家自然科学基金(31600238) （31600238）
湖南省教育厅科学研究项目(16C0769) （16C0769）
湖南省研究生科研创新项目(CX2014B302) （CX2014B302）
湖南省科技重点计划(2016SK3002) （2016SK3002）
