中国现代中药2017，Vol.19Issue(10)：1387-1390,1414,5.DOI:10.13313/j.issn.1673-4890.2017.10.007
在模拟人工胃肠道环境中博落回主要生物碱类成分的稳定性特征分析
Stability Analysis of Major Alkaloids in Macleaya cordata under Simulated Gastrointestinal Environment
摘要
Abstract
Objective:In order to explain the reasons of low bioavailability of four alkaloids (protopine,allocrypto pine,sanguinarine,chelerythrine) in Macleaya cordata,the study explored the stability of four alkaloids in the simulated gastrointestinal environment.Methods:A high performance liquid chromatography method was used to determine the stability of four alkaloids in M.cordata under the environment of different pH media,artificial gastric juice (pH =1.3) and artificial intestinal juice (pH =6.8).Results:The recovery rate of protopine and allocryptopine were ranging from 97.16％ to 101.89％ in the environment of acidic,neutral,alkalinity media,artificial gastric juice and artificial intestinal juice for 8 h.The recovery rate of sanguinarine was 48.81％-60.53 ％ in the environment of pH =6.8 phosphate buffer solution (PBS),water,pH =7.8-8.0 PBS and artificial intestinal juice for 8 h.The recovery rate of chelerythrine was 81.21％-93.48％ in the environment of pH =5.8 PBS,pH =6.8 PBS,water and artificial intestinal juice for 8 h,while in the environment of pH =7.8-8.0 PBS for 8 h,the recovery rate of chelerythrine was 57.43％.Conclusion:The stability of protopine and allocryptopine was not affected by pH.While along with the increase of pH,the stability of sanguinarine and chelerythrine was reduced.The stability of four alkaloids in M.cordata under the simulated gastrointestinal environment accorded with the environment of different pH media.The results provide a scientific basis for clarifying the pharmacokinetic characteristics of Sangrovit.关键词
博落回/血根碱/白屈菜红碱/胃肠道稳定性Key words
Macleaya cordata/sanguinarine/chelerythrine/gastrointestinal tract stability引用本文复制引用
张换成,陈梅,柳亦松,唐昭山,曾建国,刘兆颖..在模拟人工胃肠道环境中博落回主要生物碱类成分的稳定性特征分析[J].中国现代中药,2017,19(10):1387-1390,1414,5.基金项目
湖南省科技重点计划(2016SK3002) （2016SK3002）
湖南省自然科学基金(2017JJ1017) （2017JJ1017）
