川北医学院学报2017，Vol.32Issue(6)：816-820,830,6.DOI:10.3969/j.issn.1005-3697.2017.06.002
辛伐他汀调控RhoA对血管平滑肌细胞增殖与迁移及纤溶活性的影响
Effects of simvastatin on cell proliferation and migration ability of HAVSMCs by regulating RhoA-induced fibrinolysis
摘要
Abstract
Objective:To investigate the effect and mechanism of different concentrations of simvastatin on the proliferation and migration of human vascular smooth muscle cells (HAVSMCs) by regulating RhoA/ROCK-induced expression changes of fibrinolytic activity (t-PA/PAI-1).Methods:Cultured HAVSMCs were treated with different concentrations of simvastatin (0.1 μmol/L、1 μmol /L、10 μmol /L),and then cell proliferation and cell migration were measured by CCK-8 assay and wound healing test.The expression levels of RhoA/ROCK,t-PA/PAI-1 mRNA or protein activity were detected by RT-qPCR and ELISA.Results:The proliferation and cell migration of HAVSMCs were dramatically inhibited by simvastatin in a dose-dependent manner.After HAVSMCs were treated with 10 μmol/L simvastatin for 24 h,simvastatin could significantly inhibit the RhoA/ROCK signalling pathway and induce efficient inhibition of PAI-1 mRNA and protein production levels and the promotion of t-PA mRNA and protein production levels.Conclusion:Simvastatin can significantly promote fibrinolytic activity and inhibit the proliferation and cell migration of HAVSMCs through the inhibition of the RhoA/ ROCK signalling pathway,thereby suppressing PAI-1 mRNA and protein levels and increasing t-PA mRNA and protein levels.关键词
辛伐他汀/人血管平滑肌细胞/小G蛋白RhoA及下游激酶ROCK/组织纤溶酶原激活物和纤溶酶原激活物抑制剂-1/细胞增殖和迁移Key words
Simvastatin/HAVSMCs/RhoA/ ROCK signalling pathway/t-PA/PAI-1/Cell proliferation and cell migration分类
医药卫生引用本文复制引用
黄纪卫,覃数,张冬颖..辛伐他汀调控RhoA对血管平滑肌细胞增殖与迁移及纤溶活性的影响[J].川北医学院学报,2017,32(6):816-820,830,6.基金项目
重庆市自然科学基金[渝发科技字(2004) 54号文] （2004）
