重庆医学2018，Vol.47Issue(2)：145-148,4.DOI:10.3969/j.issn.1671-8348.2018.02.001
外源性NPM突变蛋白对AKT细胞定位的影响及其对白血病增殖的意义
Effect of exogenous NPM mutant protein on AKT cellular localization and its significance in leukemia proliferation
摘要
Abstract
Objective To investigate the effect of exogenous NPM mA to the subcellular localization of AKT and its significance in the proliferation in leukemia cells.Methods The co-immunoprecipitation assay was used to detect the interaction between NPM mA and AKT.The subcellular localization of NPM mA and AKT was observe by using the immunofluorescence experiment.The cells proliferation potential was assessed by CCK-8 assay.Results NPM mA could be combined with AKT in K562 cells.The immunofluorescence showed that NPM mA was mainly distributed in the cytoplasm.After co-tranfecting NPM mA and AKT into HEK293T cells,AKT was transformed from the dispersive distribution to cytoplasma.Additionally,the in vitro proliferation ability at 72 h in the K562 group was significantly enhanced compared with the K562 c1 and K562 mA groups(P＜0.01).After treating with AKT inhibitor(AKT inhibitor Ⅳ,AKT Ⅳ) for 48 h,the proliferation in the K562 c1 and K562 wt groups was remarkably inhibited,but which in the K562 mA group could still maintain growth(P＜0.01).Conclusion Exogenous NPM mA can change the subcellular localization of AKT,moreover regulates the in vitro malignant proliferation of leukemic cells.关键词
白血病/蛋白激酶类/核仁磷酸蛋白/细胞定位Key words
leukemia/protein kinases/nucleophosmin/cell localization分类
医药卫生引用本文复制引用
全静,王春,李宝林,刘靳波..外源性NPM突变蛋白对AKT细胞定位的影响及其对白血病增殖的意义[J].重庆医学,2018,47(2):145-148,4.基金项目
西南医科大学附属医院项目(2015-QS-008) （2015-QS-008）
四川省泸州市科技局(15193) （15193）
四川大学与泸州市人民政府战略合作项目(2013CDLZ-S15) . （2013CDLZ-S15）
