重庆医学2018，Vol.47Issue(2)：156-160,5.DOI:10.3969/j.issn.1671-8348.2018.02.004
Tubacin对大鼠骨髓间充质干细胞膜表面结构及力学性能的影响
Effects of tubacin on membrane surface structure of BMSCs and mechanical property
摘要
Abstract
Objective To study the effect different concentrations of HDAC6 inhibitor tubacin on the proliferation,morphology and membrane surface ultrastructure of bone marrow mesenchymal stem cells(BMSCs).Methods Primary BMSCs were cultured.The P4 generation cells were taken for conducting the experiment.The different concentrations of tubacin were used to treat the cells fro 24 h.The cells survival rate was detected by MTT assay.The atomic force microscopy(AFM) was applied to observe the cellular morphology and surface ultramicrostructure and detect the mechanical property in different groups.Results The MTT results showed that low concentration of tubacin had the effect for promoting BMSC proliferation;the AFM results showed that compared with the control group,the height and width of BMSCs after treating by low concentration of tubacin,the membrane surface roughness was decreased and cellular hardness was increased.Conclusion Low concentration of tubacin can promote the BMSC proliferation,causes the changes of morphology and membrane surface ultramicrostructure,enhances the mechanical property and increases the cell implantation treatment efficiency.关键词
间质干细胞/显微镜检查,原子力/力学性能/超微结构Key words
mesenchymal stem cells/microscopy, atomic force/mechanics/ultramicrostructure分类
医药卫生引用本文复制引用
卢芳,周艳芳,彭新生,张琛琛,梁嘉强..Tubacin对大鼠骨髓间充质干细胞膜表面结构及力学性能的影响[J].重庆医学,2018,47(2):156-160,5.基金项目
广东省科技计划项目(2015A020214020). （2015A020214020）
