重庆医学2018，Vol.47Issue(2)：220-222,3.DOI:10.3969/j.issn.1671-8348.2018.02.025
毛细管区带电泳测定大黄提取物中有效成分的含量
Measurement of effective constituents contents in rhubarb by capillary electrophoresis
摘要
Abstract
Objective To adopt the capillary electrophoresis(HPCE) for detecting the contents of emodin,chrysophanol and rhein in the extract of rheum officinale.Methods The detection was executed with a fused-silica capillary column(67.4 cm× 75.0 μm,effective length 51.0cm) as the separation column.The buffer solution consisted of 60 mmol/L Na2B4O7,40 mmol/L Na2CO3 and 40 mmol β-cyclodextrin(pH 8.9).The detection wavelength was 254 nm.Results RSD of precision in emodin,chrysophanol and rhein was 1.79％,4.46％ and 2.30％.respectively The linear range of 3 components was 1.1-19.0 mg/mL;the average recovery rates of emodin,chrysophanol and rhein were 100.14 ％,99.65 ％ and 98.44 ％ respectively,RSD was 2.43 ％,2.54 ％ and 2.02％ respectively(n=3).Conclusion The method can be used in the content determination of rheum officinale extract.关键词
电泳,毛细管/大黄/含量测定Key words
electrophoresis, capillary/rheum officinale/content determination分类
医药卫生引用本文复制引用
曾雪,杨元娟,陈竹,刘应杰,夏培元..毛细管区带电泳测定大黄提取物中有效成分的含量[J].重庆医学,2018,47(2):220-222,3.基金项目
重庆市科技委员会基础与前沿项目(cstc2014jcyjA10125) （cstc2014jcyjA10125）
重庆市卫生和计划生育委员会中医项目(zy20150242) （zy20150242）
重庆市卫生和计划生育委员会医学项目(2012-1-093) （2012-1-093）
重庆市教育委员会科技项目(KJ132501) （KJ132501）
重庆医药高等专科学校校级重点项目(ygz2015101). （ygz2015101）
