解放军医药杂志2018，Vol.30Issue(1)：38-41,4.DOI:10.3969/j.issn.2095-140X.2018.01.010
转染HBx改变Notch信号诱导肝癌HepG2细胞株多药耐药性研究
A Study on Multidrug Resistance of Cell Line HepG2 induced by Changing Notch Signaling Pathway through HBx Transfection in Hepatocellular Carcinoma
摘要
Abstract
Objective To investigate incidence mechanism of multidrug resistance induced by affecting Notch signaling pathway through HBx transfection in hepatocellular carcinoma ( HCC ) . Methods Human hepatoma HepG2 cell line was transfected, and the samples were divided into transfected HBx cell group ( HepG2-HBx group) , transfected cell group with empty vector ( HepG2-con group) and blank cells group ( HepG2 group) . In 3 groups, HBx mRNA ex-pressions were detected by quantitative polymerase chain reaction ( PCR) , and HBx, Notch-1 and multidrug resistance-associated proteins ( MRP) expressions were detected by Western-blot method, and cell conditions of proliferation activity and multidrug resistance were detected by CCK-8. Results HBx fragments and HBx expressions were detected in HepG2-HBx group, while no HBx mRNA and protein expressions could be found in HepG2-con and HepG2 groups. Compared with those in HepG2-con and HepG2 groups, cell proliferation activity was significantly enhanced, and Notch-1 and multidrug resistance-associated proteins ( MRP ) protein expressions were significantly increased in HepG2-HBx group (P<0. 05). Cell cycle detection showed that in HepG2-HBx group, cell number in G0/G1 phase was significantly decreased, while cell number in S phase was significantly increased compared with those in HepG2-con and HepG2 groups (P<0. 05, P<0. 01). Multidrug resistance (MDR) examination showed that resistance indexes for 5 drugs were significantly increased in HepG2-HBx group (P <0. 05). Conclusion HepG2 cell line in hepatocellular carcinoma transfected by HBx can finally lead to incidence of increasing HepG2 proliferation and multidrug resistance through indu-cing Notch-1 and MRP overexpressions.关键词
肝肿瘤/HepG2细胞/HBx/转染/Notch信号通路/多药耐药相关蛋白质类Key words
Liver neoplasms/HepG2 cells/HBx/Transfection/Notch signaling pathway/Multidrug resistance-associated proteins分类
医药卫生引用本文复制引用
徐桐红,于冬艳,任玲..转染HBx改变Notch信号诱导肝癌HepG2细胞株多药耐药性研究[J].解放军医药杂志,2018,30(1):38-41,4.基金项目
辽宁省自然科学基金项目(2014020101) （2014020101）
