河北医学2018，Vol.24Issue(1)：1-5,5.DOI:10.3969/j.issn.1006-6233.2018.01.001
阿托伐他汀预诱导HO-1表达对泛影葡胺致大鼠急性肾损伤模型保护性作用研究
Protective Effect of Atorvastatin Preinduced HO-1 Expression on Acute Renal Injury Induced by Meglumine in Rats
摘要
Abstract
Objectives:To investigate whether atorvastatin can induce heme oxygenase-l ( HO-1) ex-pression in rat kidneys to prevent contrast-induced acute kidney injury( CI-AKI) and its related mechanisms. Methods:48 healthy Sprague-Dawley male rats were randomly divided into the blank control group, model group , atorvastatin group and zinc protoporphyrin IX(ZnPPIX) group. A rat model of CI-AKI was estab-lished by intramuscular injection of gentamicin sulfate for 2 days and then intravascular injection of 76％meglumine diatrizoate. Atorvastatin group and ZnPPIX group were pretreated with atorvastatin/ZnPPIX before using meglumine diatrizoate. We detected changes in serum creatinine ( Scr) before and after model establish-ment, measured the interleukin-6 ( IL-6 ) , monocyte chemotactic protein 1 ( MCP-1 ) , malondialdehyde ( MDA) and total antioxidant capacity ( T-AOC) as well as the protein expression of HO-1, Bax, Bcl-2 in the renal tissue, and analyzed the renal cell apoptosis index ( AI ) . Results:After contrast agent injection, compared with the control group,the SCr of the other three groups were significantly increased and reached the CI-AKI standard, Meanwhile, the inflammatory and oxidative stress markers and AI were also significantly in-creased. Compared with the model group, in atorvastatin group, the expression of HO-1 protein in renal tissue increased significantly, while the level of inflammation and oxidative stress and both AI and SCr decreased significantly ( P<0.05) ,in ZnPPIX group, the expression of HO-1 protein in renal tissue also increased, but the level of inflammation and oxidative stress and both AI and SCr increased significantly( P<0.05) . Conclusion:Atorvastatin can induce expression of HO-1 protein in rat kidney throuh which inhibit the renal inflammation and oxidative stress and cell apoptosis, thereby protecting against CI-AKI.关键词
阿托伐他汀/血红素氧化酶-1/大鼠Key words
Atorvastatin/Heme oxygenase-1/Rat引用本文复制引用
魏开勇,王忠庆,黄维义..阿托伐他汀预诱导HO-1表达对泛影葡胺致大鼠急性肾损伤模型保护性作用研究[J].河北医学,2018,24(1):1-5,5.基金项目
四川省卫生厅资助项目,(编号:090216) （编号:090216）
