四川大学学报（医学版）2018，Vol.49Issue(1)：8-12,5.
石榴叶提取物诱导前列腺癌细胞凋亡并抑制其增殖转移的研究
The Extract from Punica Granatum (Pomegranate) Leaves Promotes Apoptosis and Impairs Metastasis in Prostate Cancer Cells
邓远乐 1李亚丽 1郑婷婷 1户明星 2叶庭洪 2谢永美 2阴文娅1
作者信息
- 1. 四川大学华西公共卫生学院营养与食品卫生毒理系 成都610041
- 2. 四川大学华西医院生物治疗国家重点实验室 成都610041
- 折叠
摘要
Abstract
Objective To investigate the effects of pomegranate leaves extract (PLE)on proliferation,apoptosis and metastasis of prostate cancer cells.Methods The proliferation of TRAMP-C1,DU145,PC3 prostate cancer cells treated with different concentrations of PLE (final mass concentrations were 12.5,25,50, 100,200 μg/mL,respectively) for different time (24,48,72 h) was detected by MTT assay.Colony formation assay was performed to verify the long-term effects of PLE on the proliferation of DU145 and PC3 cells.After being treated with PLE for 48 h,Hoechst-33258 staining was used to observe the changes in the nucleus,the cell apoptotic rate was detected by flow cytometry,and wound-healing migration assay was perform to test the change of migration.Results In comparison with the control group,PLE in the range of 12.5-200 μg/mL had a certain inhibitory effect on the proliferation of TRAMP-C1,DU145 and PC3 cells (P＜0.05).In the range of 6.25-100 μg/mL,the number of colony formation of DU145 and PC3 was significantly reduced(P＜0.01).After PLE treated for 48 h,the apoptotic features of nuclear fragmentation and the formation apoptotic body was observed in PC3.With the increase of concentration,the apoptotic rate increased gradually (P＜0.05),and the ability of cells to migrate to the scratch area was significantly weaker than the control group (P＜0.01).Conclusion PLE has effect on proliferation,apoptosis and metastasis of prostate cancer cells.关键词
石榴叶提取物/前列腺癌/PC3/凋亡Key words
Pomegranate leaves extract (PLE)/Prostate cancer/PC3/Apoptosis引用本文复制引用
邓远乐,李亚丽,郑婷婷,户明星,叶庭洪,谢永美,阴文娅..石榴叶提取物诱导前列腺癌细胞凋亡并抑制其增殖转移的研究[J].四川大学学报（医学版）,2018,49(1):8-12,5.
