四川大学学报（医学版）2018，Vol.49Issue(1)：29-33,58,6.
缺氧诱导因子及其抑制因子调控肾癌细胞生长的实验研究
Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells
摘要
Abstract
Objective To detect the expression and growth regulation effects of the factor inhibiting hypoxiainducible factor (FIH-1) in renal carcinoma cells.Methods The expressions of FIH-1,hypoxia inducible factor (HIF) and vascular endothelial growth factor (VEGF) were examined in OS-RC-2 and 786-0 cell lines via real-time PCR (RT-PCR) and Western blot.Overexpression plasmid of FIH-1 were designed and constructed as EX-V1104-M45-FIH-1.After the transfection of the plasmid into OS-RC-2 and 786-0 cells,the expression levels of FIH-1,HIF and VEGF were measured by RT-PCR and Western blot,while cell proliferation was tested by MTT.Results The expressions of FIH-1,HIF-1α,HIF-2α and VEGF were detected in OS-RC-2 cells,and FIH-1,HIF-2α and VEGF were detected in 786-0 cells.After the transfection of EX-V1104-M45-FIH-1,the level of FIH-1 was increased in OS-RC-2,while the levels of HIF-2α,HIF-1α and VEGF were decreased.MTT showed that FIH-1 plasmid transfection decreased the cell proliferation of OS-RC-2,but did not show inhibition on 786-0 cells proliferation.Conclusion FIH-1/HIF-1α/VEGF signaling pathway exists in renal carcinoma cell lines.关键词
肾细胞癌/缺氧诱导因子抑制因子/缺氧诱导因子/RNA干扰Key words
Renal cell carcinoma/Factor inhibiting hypoxia-inducible factor-1/Hypoxia inducible factor/RNA interference引用本文复制引用
邓实,杨博,任正举,董强..缺氧诱导因子及其抑制因子调控肾癌细胞生长的实验研究[J].四川大学学报（医学版）,2018,49(1):29-33,58,6.基金项目
四川省科技厅科研课题(No.2014SZ0210、No.2017SZ0149)、四川省卫生厅科研课题(No.YN20140030)和四川省中医药管理局课题(No.2016Q042)资助 （No.2014SZ0210、No.2017SZ0149）
