家畜生态学报2017，Vol.38Issue(12)：11-17,7.
Tektin3基因在猪生殖系统发育中的表达规律研究
Cloning and Expression of Tektin3 Gene in Swine Reproductive System Development
摘要
Abstract
TEKTINS were essential for construction of axoneme in sperm flagella.Tektin3 is one member of TEKTINs family.This study aimed to study the expression of Tektin3 gene in Meishan pigs.The cDNA of Tektin3 gene was cloned with RACE to get its sequence and its structures were analyzed by bioinformatics.The mRNA distribution of the porcine Tektin3 was analyzed in 23 tissues of Meishan boars and sows with Semi-quantitative PCR.The expression of Tektin3 was analyzed in testis of 2-day,30-day-old,60-day-old,90-day-old,150-day-old Meishan boars with Realtime PCR.The result showed as follows:(1) The full length sequence of Tektin3 was 1 761 bp in length,containing 95 bp 5’ UTR and coding region (CDS,1 473 bp) and 193 bp 3’ UTR and encoding 490 amino acids.The isoelectric point (PI) of the protein was 7.94,and the molecular weight was 56 481.33.(2)The expression level of porcine Tektin3 was significant in testis but not significant in hypothalamus and uterinehorn.(3) Tektin3 begun to express in 60-day-old testis,but a significant drop in 90 days(P＜0.05),a significant increase in 150 days(P＜0.05).It implied that besides the function on sperm tail structural protein,the Tektin3 may be involved in structure of cilia of some organs or tissues and sexual maturity of boars.关键词
梅山猪/Tektin3/基因克隆/生物信息学/时空表达Key words
Meishan pig/Tektin3/gene cloning/sequence analysis/temporal and spatial expression分类
农业科技引用本文复制引用
王宵燕,陈子璇,张亚妮,宋成义,李碧春..Tektin3基因在猪生殖系统发育中的表达规律研究[J].家畜生态学报,2017,38(12):11-17,7.基金项目
江苏省科技厅自然科学基金青年基金项目(BK20150440) （BK20150440）
江苏高校优势学科建设工程资助项目 （）
