家畜生态学报2017，Vol.38Issue(12)：43-47,5.
L-肉毒碱对牛精液体外保存时间及品质的影响
Effect of L-carnitine on Sperm Quality and Storage Time of Bovine Semen in Vitro
摘要
Abstract
In vitro incubation is known to decrease bovine sperm quality.L-carnitine (LC),a kind of antioxidant,facilitates the transport of activated fatty acids from the cytosol to the mitochondrial matrix.It can reduce reactive oxygen species (ROS) level and the occurrence of lipid peroxidation.In this study,the effect of LC concentration (0,2,10,15 mM) and incubation time (2,4,6 h) on bovine sperm progressive motility,plasma membrane integrity,mitochondrial activity and acrosome integrity was investigated.The result showed that progressive motility (45.6％,42.4％ vs 33.0％),the percentage of sperm with plasma membrane integrity (73.6％,68.4％ vs 61.2％) and acrosome integrity (88.4％,86.3％ vs 81.4％) of the bovine sperm were higher (P＜0.05) for 10 mM and 15 mM LC than those of control,however,mitochondrial activity was the best for 10 mM LC.Furthermore,the progressive motility,the percentage of spermatozoa with plasma membrane integrity and high mitochondrial membrane potential in 10 mM LC groups at 2 h,4 h,6 h were higher than that in the control (P＜0.05).There wasn't significant difference between 10 mM LC and the control on acrosome integrity after 6 h incubation (P＞0.05).In conclusion,L-carnitine provided a protection for sperm against in vitro storage-induced functional and structural damages,and improved sperm quality parameters.Meanwhile,it also increased storage time for bovine semen in vitro.关键词
L-肉毒碱/牛/精液品质/体外保存Key words
L-carnitine/bovine/semen quality/in vitro preservation分类
农业科技引用本文复制引用
李晓霞,曹平华,韩文霞,禹学礼..L-肉毒碱对牛精液体外保存时间及品质的影响[J].家畜生态学报,2017,38(12):43-47,5.基金项目
国家自然科学基金项目(31201801) （31201801）
河南省自然科学基金项目(162300410080) （162300410080）
河南省高等学校重点科研项目计划(15A230005) （15A230005）
河南科技大学博士科研启动基金(09001551) （09001551）
