家畜生态学报2018，Vol.39Issue(1)：14-18,5.
不同产羔数小尾寒羊FOXL2和FIGLA基因的表达研究
Study on the Expression of FOXL2 and FIGLA Genes in Small Tail Han Sheep with Different Litter Size
摘要
Abstract
In order to explore the association of FOXL2 and FIGLA with litter size in sheep,real-time PCR was performed to detect FOXL2 and FIGLA mRNA expression in eight important reproduction related tissues of both multiparous and uniparous Small Tail Han ewes.The results showed that the expression of FOXL2 and FIGLA was at high level in ovary of both multiparous and uniparous Small Tail Han Sheep,followed by hypothalamus,pituitary,oviduct,uterus body and uterin horn.The expression was relatively low in brain and cerebellum.The expression of FOXL2 in cerebellum and uterus body in multiparous sheep was significantly higher than that in uniparous sheep (P＜0.05).The expression of FOXL2 in pituitary and ovary in multiparous sheep was significantly lower than that in uniparous sheep (P＜0.05).The expression of FOXL2 in hypothalamus in multiparous sheep was significantly lower than that in uniparous sheep (P＜0.01).The expression of FIGLA in brain of multiparous sheep was significantly higher than that of uniparous sheep (P＜0.05),and there were no significant differences in other tissues between multiparous and uniparous sheep (P＞0.05).This study preliminarily indicated that the FOXL2 expression may influence sheep litter size to some extent,while the FIGLA expression may have no effect on sheep litter size.关键词
绵羊/FOXL2/FIGLA/组织表达Key words
sheep/FOXL2/FIGLA/tissue expression分类
农业科技引用本文复制引用
周梅,刘秋月,储明星,曹晓涵,贺小云,孙庆,狄冉,胡文萍,王翔宇,张效生,张金龙..不同产羔数小尾寒羊FOXL2和FIGLA基因的表达研究[J].家畜生态学报,2018,39(1):14-18,5.基金项目
国家自然科学基金项目(No.31402041,No.31472078) （No.31402041,No.31472078）
国家转基因科技重大专项(No.2016ZX08009-003-006,No.2016ZX08010-005-003) （No.2016ZX08009-003-006,No.2016ZX08010-005-003）
国家肉羊产业技术体系专项(No.CARS-38) （No.CARS-38）
中央级公益性科研院所基本科研业务费专项(No.Y2017JC24,No.2017ywf-zd-13,No.2013ywf-zd-1,No.2015ywf-zd-2,No.2015ywf-zd-8) （No.Y2017JC24,No.2017ywf-zd-13,No.2013ywf-zd-1,No.2015ywf-zd-2,No.2015ywf-zd-8）
中国农业科学院科技创新工程(No.ASTIP-IAS13,No.CAAS-XTCX2016010-01-03,No.CAAS-XTCX2016010-03-03,No.CAAS-XTCX2016011-02-02) （No.ASTIP-IAS13,No.CAAS-XTCX2016010-01-03,No.CAAS-XTCX2016010-03-03,No.CAAS-XTCX2016011-02-02）
宁夏农林科学院科技创新先导资金项目(No.DWJLC-2016001) （No.DWJLC-2016001）
内蒙古自治区科技重大专项 （）
农业科研杰出人才及其创新团队项目 （）
国家万人计划科技创新领军人才项目 （）
天津市科技计划项目(16ZXZYNC00050) （16ZXZYNC00050）
