家畜生态学报2018，Vol.39Issue(1)：64-68,83,6.
新疆石河子地区犬源立克次体病流行病学调查
Survey on Molecular Epidemiology of Rickettsiales in Dogs in Shihezi Xinjiang
摘要
Abstract
Rickettsia,Anaplasma and Ehrlichia of the order Rickettsiales can cause rickettsiosis,anaplasmosis,and ehrlichiosis,respectively.Rickettsiales bacteria are found in ticks worldwide.Ixodes is the major vector.The aim of this research was to screen,identify and characterize Rickettsia,Anaplasma and Ehrlichia by PCR and phylogenetic analysis in dogs.The parasite ticks and blood in dogs were collected from the pet hospitals in Shihezi City and its surrounding area.Rickettsia,Anaplasma and Ehrlichia cani 16S rRNA were detected by PCR and compared with the corresponding genes in GenBank.The 345 bp specific fragment of Rickettsia was amplified from ticks,and the specific fragments of 452 bp of Ehrlichia cani and Anaplasma were obtained.The sequencing results confirmed that these fragments were Rickettsia,Anaplasma and Ehrlichia cani,phylogenetic analyses of these products showed that canine Rickettsia,Ehrlichia and Anaplasma isolates in the study classified in the same branch with Rickettsiales bacterium (EF687768),Ehrlichia sp.Yonaguni206 (HQ697589.1),Anaplasma (KJ410247.1,KJ410248.1,KJ410249.1) and belong to the conservative clade of Rickettsia,Ehrlichia and Anaplasma,respectively,suggesting a close relationship with these foreign strains.The purpose of this study was to investigate the infection and prevalence of tick borne Rickettsia in dogs in Shihezi,and to provide epidemiological data for the prevention and treatment of rickettsiosis.关键词
立克次体/无形体/犬埃立克体/蜱/16S rRNAKey words
Rickettsia/Anaplasma/Ehrlichia cani/tick/16S rRNA分类
农业科技引用本文复制引用
李重阳,孟庆玲,乔军,伍晔晖,王立霞,郭晶,马帅,才学鹏..新疆石河子地区犬源立克次体病流行病学调查[J].家畜生态学报,2018,39(1):64-68,83,6.基金项目
国家十三五重点研发计划子课题(No.2016YFD0501005) （No.2016YFD0501005）
兵团中青年科技创新领军人才计划(2016BC001) （2016BC001）
