日用化学工业2018，Vol.48Issue(1)：1-7,7.DOI:10.13218/j.cnki.csdc.2018.01.001
钙离子与碱性蛋白酶的相互作用及机理研究
Interaction between calcium ions and alkaline protease and study of its mechanism
摘要
Abstract
The interaction of calcium ions and alkaline protease was studied. The mechanism of action was investigated by dynamic light scattering (DLS)and fluorescence spectroscopy. Results show that calcium ions have a certain activation effect on the activity of alkaline protease,in which calcium chloride activates the activity of alkaline protease more significantly than calcium formate. DLS experiments show that calcium ion and alkaline protease are mainly combined by electrostatic force. With the addition of calcium ions,the average particle size of the alkaline protease molecules decreased,and the hydrophobic structure gradually shift to the interior of the enzyme molecule,which is beneficial to the improvement of alkaline protease activity. The binding of calcium ions to alkaline protease was studied by fluorescence spectroscopy. Results show that the quenching mechanism of calcium ions to alkaline protease is a static quenching. Calcium ions form a stable complex with alkaline protease. The binding ability is strong,and the process can be spontaneous.关键词
碱性蛋白酶/钙离子/活性/动态光散射/荧光光谱Key words
alkaline protease/calcium ion/activity/dynamic light scattering/fluorescence spectroscopy分类
化学化工引用本文复制引用
李静,张剑,赵永祥..钙离子与碱性蛋白酶的相互作用及机理研究[J].日用化学工业,2018,48(1):1-7,7.基金项目
山西省重点研发计划国际合作项目(201603D421029) （201603D421029）
