日用化学工业2018，Vol.48Issue(1)：57-60,4.DOI:10.13218/j.cnki.csdc.2018.01.012
高效液相色谱法测定化妆品中氨甲环酸的含量
Determination of the content of tranexamic acid in cosmetics by high performance liquid chromatography
唐毓萍 1唐欣 1孙培文 1林艺青 1李征1
作者信息
- 1. 上海中翊日化有限公司,上海 200065
- 折叠
摘要
Abstract
A high performance liquid chromatography was used to determine the content of tranexamic acid in cosmetics. The Zorbax SB C18 chromatographic column (250 mm × 4. 6 mm,5 μm)was used for separation, and water-methanol-18. 3 g/L sodium dihydrogen phosphate solution (containing volume fraction 1. 26％triethylamine and adjusted pH to 2. 5 by phosphoric acid)was used as mobile phase for gradient elution,with a flow rate of 0. 8 mL/min. Temperature of the column was 25℃ and the injection volume was 10μL. Samples were extracted with methanol/saturated sodium chloride solution (volume ratio of 1:1 )and quantitated by diode array detector at 220 nm with external standard method. Results show that tranexamic acid has a good linear relationship in the range of 100-4000 mg/L,and the correlation coefficient (R2 )is 0. 9999. Limit of detection and quantitation of the method are 7 and 22 mg/L separately. The average spiked recoveries vary from 94. 0％ to 102. 5％,and the relative standard deviations achieve 0. 57％ -2. 69％(n=3). This method has the advantages of simple pretreatment,high recovery and good precision for the determination of the content of tranexamic acid in cosmetics.关键词
化妆品添加剂/高效液相色谱/氨甲环酸Key words
additive of cosmetics/high performance liquid chromatography/tranexamic acid分类
化学化工引用本文复制引用
唐毓萍,唐欣,孙培文,林艺青,李征..高效液相色谱法测定化妆品中氨甲环酸的含量[J].日用化学工业,2018,48(1):57-60,4.
