山东中医药大学学报2018，Vol.42Issue(1)：74-77,4.DOI:10.16294/j.cnki.1007-659x.2018.01.024
五味子醇甲对大鼠离体胸主动脉环作用研究
Vasorelaxation Effect of Schisandrol A on Isolated Thoracic Aortic Rings in Rats
摘要
Abstract
Objective:To investigate the effects of Schisandrol A on isolated thoracic aortic rings in Wistar rats and study the potential mechanism. Methods:Isometric tension recording was applied to evaluate the effect of cumulative Schisandrol A on endothelium-intact/denuded aorta rings under quiescent condition or pre-contracted with phenylephrine ( PE , 1 × 10-6 mol/L ) . The effect of Schisandrol A at the aforementioned doses on endothelium-intact aortic rings,pre-inhibited separately with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester(L-NAME,3×10-4 mol/L),guanylate cyclase inhibitor(ODQ,1×10-5 mol/L), cyclooxygenase inhibitor indomethacin(INDO,1×10-5 mol/L),contracted with PE was also observed. And the effects of PE-induced Ca2+ release and CaCl2-induced Ca2+ influx were observed. Results:Under the condi-tion of no change of resting tension,Schisandrol A had a significant effect on endothelium-intact and en-dothelium-denuded thoracic aortic rings which were pre-contracted with PE and there was statistical signifi-cance between the two groups(P<0.05). Compared with the non-blocking group,there were statistical signifi-cance after incubation with L-NAME(P<0.05) and ODQ(P<0.05). The relaxation rate of the rings incubated with INDO was not statistical significance from that in the non-blocking group(P>0.05). Schisandrol A ob-viously inhibited the contraction induced by CaCl2. Conclusions:Schisandrol A can inhibit the vasoconstric-tion caused by PE in a dose-dependent manner,and it was endothelium-dependent and non-endothelium-de-pendent. The endothelium-dependent mechanism may be related to the NO-cGMP pathway,and the non-en-dothelium-dependent mechanism may be associated with inhibition of receptor-dependent calcium channels on the vascular smooth muscle,which leads to the de-crease of Ca2+ influx.关键词
五味子醇甲/胸主动脉环/舒张/NO/Ca2+/大鼠Key words
Schisandrol A/thoracic aotic ring/vasor-elaxation/NO/Ca2+/rat分类
医药卫生引用本文复制引用
孔李婷,黄颖,翟华强,袁一平,郭兆娟,贾晓玉..五味子醇甲对大鼠离体胸主动脉环作用研究[J].山东中医药大学学报,2018,42(1):74-77,4.基金项目
国家自然科学基金项目(编号:81373887) （编号:81373887）
