上海海洋大学学报2018，Vol.27Issue(1)：48-54,7.DOI:10.12024/jsou.20170402003
人工诱导日本鳗鲡成熟过程中血清及性腺内类固醇激素含量的变化
The determination of steroid hormones in Anguilla japonica gonad and blood during the artificial induction of maturation by UPLC-MS/ MS
摘要
Abstract
With the aim to investigate the changes of steroid hormones in the serum and gonadal tissue of Japanese eel(Anguilla japonica)during artificial induction of maturation,we have developed and validated a UPLC-MS / MS method to determine 6 steroids in serum samples simultaneously,including testosterone(T), estrone(E 1 ),17α-hydroxyprogesterone(17α-OHP),17α,20β-dihydroxy-4-pregnen-3-one(DHP),estradiol-17β(E 2 ),estriol(E 3 ),and 4 steroids (T,DHP,E 2 ,E 3 )in gonadal tissue. The results showed that the average recoveries in serum and gonadal tissue detection were 86. 9％ - 98％ and 75. 6％ - 88. 1％, respectively;and the detection limit(S / N > 10)were 0. 1ng / g and 0. 1 ng / mL. The steroid hormones in serum and gonad tissue of experimental groups were increased through the hormone injection. Except for the content of E3 ,all the other five steroid hormones reached their highest content during spawning,however,the E2 and DHP in gonadal tissue of experimental groups reached the highest content during dystocia. Conversely, not any of E1 and E3 in serum,as well as E 2 and E3 in gonadal tissue were detected through the period in the control group. These results provide preliminary understanding of sex steroid hormones changes during the maturation of Anguilla japonica,moreover,it established a sensitive and stable method to determine the sex steroid hormones in aquatic animals.关键词
类固醇激素/日本鳗鲡/超高效液相色谱-串联质谱/性腺/血清Key words
steroids/Anguilla japonica/UPLC-MS / MS/serum/gonad tissue分类
农业科技引用本文复制引用
蒲金成,湛嘉,刘登攀,杜力,黎原谷,刘利平..人工诱导日本鳗鲡成熟过程中血清及性腺内类固醇激素含量的变化[J].上海海洋大学学报,2018,27(1):48-54,7.基金项目
中国-东盟海上合作基金项目(DF) （DF）
