天然产物研究与开发2017，Vol.29Issue(12)：2092-2096,5.DOI:10.16333/j.1001-6880.2017.12.016
正交实验法优化黄柏中黄连素的酶碱法提取工艺
Optimization of Enzyme-alkali Method Extraction Process of Berberine from Cortex Phellodendri by Orthogonal Experiments
摘要
Abstract
The objective of this study was to find out the optimal extraction conditions of berberine from Cortex Phellodendri by applying the enzyme-alkali technology.Based on the single factor experiments,the orthogonal test was designed to optimize the Enzyme-alkali technology to extract berberine from cortex phellodendri with the extraction ratio as evaluation index.The optimal extraction conditions were as follows:the concentration of cellulase with 15‰,enzymolysis time with 1 h,substrate/liquid with 1∶7,and extraction time with 7 h.The process was suitble for its industrialized production with high yield,low cost and good stability.关键词
正交实验/黄柏/黄连素/纤维素酶/石灰水Key words
orthogonal test/cortex phellodendri/berberine/cellulose/calcarea hydrica分类
医药卫生引用本文复制引用
王玉洁,刘静,王碧霞,徐洲,刘莉,丁春邦..正交实验法优化黄柏中黄连素的酶碱法提取工艺[J].天然产物研究与开发,2017,29(12):2092-2096,5.基金项目
四川省科技厅科技支撑计划(2014NZ0103) （2014NZ0103）
