天然产物研究与开发2017，Vol.29Issue(12)：2097-2103,2091,8.DOI:10.16333/j.1001-6880.2017.12.017
银杏叶黄酮含量对银杏叶提取物大孔树脂纯化工艺条件的影响研究
Effects of Flavonoid Content in Ginkgo biloba Leaves on Macroporous Resin Purification Process of Ginkgo biloba Extract
摘要
Abstract
This study aims to investigate the effect of flavouoids contents in Ginkgo biloba leaves on the quality,yield and flavonoid extraction rate of extract of G.biloba leaves (EGb),and provide quality standard of raw material and corresponding processing parameters for EGb production.G.biloba leaves with flavonoid content of 1.0％,0.8％ and 0.6％ were applied to study the effects of the quality of Ginkgo biloba leaves on the macroporous resin purification process for ensuring the quality of EGb to meet the requirement of Chinese Pharmacopoeia.Statistic regression was used to investigate the relationship between EGb properties and the concentration and volume of ethanol for purification,respectively.These properties include flavonoid content and yield of EGb,and extraction rate of flavonoids.The results showed that the concentration of ethanol and eluent volume was positively correlated with the content of flavonoids in EGb.The yield of EGb was negatively correlated with the eluent volume and changed with the contents of flavonoids in the leaves.The quality of EGb extracted from Ginkgo Leaves with low flavonoid concentration was most sensitive to the processing parameters.The content of lactone in EGb had a positive relation with flavonoid content in Ginkgo biloba leaves.It also had a negative correlation with the flavonoid content of EGb and eluent volume.To keep the quality,processing conditions and yield of EGb stable,it was suggested to mix G.biloba leaves with different flavonoid concentration to make the flavonoid content in the leaves be 1.0％.By using twice amount of 15％ ethanol to elute the resins,EGb containing more than 24％ of flavonoids and 6％ of lactone can be prepared with yield of 2.6％ and over 66％ flavonoid extraction rate.关键词
银杏叶提取物/银杏叶质量/黄酮醇苷Key words
Ginkgo biloba extract/Ginkgo leaves/flavonoid glycosides分类
医药卫生引用本文复制引用
倪力军,颜玮韬,张立国..银杏叶黄酮含量对银杏叶提取物大孔树脂纯化工艺条件的影响研究[J].天然产物研究与开发,2017,29(12):2097-2103,2091,8.
