天然产物研究与开发2017，Vol.29Issue(12)：2104-2111,2062,9.DOI:10.16333/j.1001-6880.2017.12.018
秦艽环烯醚萜苷类成分分离纯化工艺及抑菌活性研究
Separation, Purification and Antibacterial Activity of Iridoid glycosides from Gentiana macrophylla Pall.
摘要
Abstract
The aim of this study was to optimize the separation and purification technology of iridoid glycosides from Gentiana macrophylla Pall.with XDA-1 macroporous adsorption resin.The antibacterial activity of the purified iridoid glycosides were studied in vitro.With the content of gentiopicroside as index,dynamic adsorption-separation was used to determine the optimal separation and purification conditions of iridoid glycoside from G.macrophylla.The optimal conditions of XDA-1 macroporous adsorption resin were finally determined as follows:sample injection concentration was 0.07 g original/mL,pH was 5.0,sample flow rate was 4 BV/h,quantity of adsorption was 32 BV,desorption rate was 3 BV/h,the pH was 7.0 and elution volume was 10 BV of 50％ ethanol.The purity of obtained iridoid glycosides was 62.97％.Moreover,the antibacterial activity of the extract was evaluated by 3 bacteria inhibition zone and minimum inhibitory concentration.The results showed that the obtained iridoid glycosides had good antibacterial activity.关键词
大孔吸附树脂/秦艽/环烯醚萜苷/分离纯化/抑菌Key words
macroporous adsorption resin/Gentiana macrophylla Pall./iridoid glycosides/separation and purification/antibacterial activity分类
医药卫生引用本文复制引用
郝彩琴,冷晓红,李军,郭超,陈海燕..秦艽环烯醚萜苷类成分分离纯化工艺及抑菌活性研究[J].天然产物研究与开发,2017,29(12):2104-2111,2062,9.基金项目
宁夏回族自治区教育厅项目(NGY2015215) （NGY2015215）
