天然产物研究与开发2017，Vol.29Issue(12)：2122-2127,6.DOI:10.16333/j.1001-6880.2017.12.021
芒果苷对高糖诱导下系膜细胞自噬的影响
Effects of Mangiferin on Autophagy in Mesangial Cell with High Glucose Condition
摘要
Abstract
To study the anti-proliferative effect of mangiferin on mesangial cell with high glucose condition,and its role on mesangial cell autophagy.CCK8 method was used to detect the anti-proliferative effect of mangiferin on mesangial cell with high glucose condition.After treatment with 24 h,the expression levels of autophagy marker LC3-Ⅱ protein in these five groups were detected by western blot.The GFP-LC3 green fluorescent trace method was used to detect autophagy of mesangial cells.The results showed that compared with high glucose group,Mangiferin treatment group (5 × 10-5,1 × 10-5,5 × 10-6 mol/L) can significantly inhibit mesangial cell proliferation (P ＜ 0.01).Compared with high glucose group,LC3-Ⅱ protein expression level of mangiferin treatment group (5 × 10-5,1 × 10-5 mol/L) was higher (P ＜ 0.05).Mangiferin treatment group (1 × 10-5,2 × 10-5,4 × 10-5,8 × 10-5 mol/L),the green fluorescent dot significantly increased.Mangiferin can inhibit the proliferation of mesangial cells with high glucose condition,its mechanism may be through improving the autophagy level of mesangial cell,maintaining the stability of environment in mesangial cell.关键词
芒果苷/自噬/系膜细胞/糖尿病肾病Key words
mangiferin/autophagy/mesangial cell/diabetic nephropathy分类
医药卫生引用本文复制引用
王晓丹,高丽辉,林华,王晋文,牛艳芬,李玲..芒果苷对高糖诱导下系膜细胞自噬的影响[J].天然产物研究与开发,2017,29(12):2122-2127,6.基金项目
国家自然科学基金(81660615) （81660615）
云南省基金重点项目(2017FE467-004) （2017FE467-004）
